剪切力和ATP定量调控血管内皮细胞钙信号的数值仿真研究

Numerical simulation research on quantitative regulation of intracellular Ca^(2+) signals in vascular endothelial cells via shear stress and ATP modulation

血管内皮细胞内钙信号与细胞功能的调控密切相关。流体剪切力和三磷酸腺苷(ATP)是两种重要的细胞钙信号调节因子。该文基于剪切力和ATP诱发血管内皮细胞内钙响应的动力学数学模型,利用PID反馈控制原理和数值仿真的方法,通过控制剪切力和ATP信号对血管内皮细胞内钙信号进行了定量调控。结果表明,对于正弦波、方波以及"Liao Ning(辽宁)"首字母缩写"LN"三种不同的细胞内期望钙信号,选择合适的控制器参数,通过控制剪切力或ATP信号都可较好地实现对血管内皮细胞内钙信号的定量控制;通过控制剪切力信号比通过控制ATP信号能对血管内皮细胞内钙信号实现更为精确的定量调控。该文结果为进一步深入研究剪切力、ATP、钙信号与内皮细胞功能的定量关系提供了一定的理论可行性和方法学基础。

Intracellular calcium signals in vascular endothelial cells are closely related to the regulation of cell function. Fluid shear stress and ATP are two important regulators of intracellular calcium signals. Based on the dynamic mathematical model of intracellular calcium signals induced by shear stress and ATP, a quantitative regulation of calcium signaling in vascular endothelial cells by controlling shear stress and ATP is presented by using the PID feedback controller and the method of numerical simulation. The results show that the intracellular calcium signals in vascular endothelial cells could be quantitatively well controlled by controlling shear stress and ATP signals with appropriate controller parameters for three different types of reference intracellular calcium signals, including sinusoidal wave, square wave, and ＂LN＂, which is the acronym of ＂Liao Ning＂; More accurate and quantitative control of intracellular calcium signals in vascular endothelial cells could be implemented by controlling shear stress signals than ATP signals. The results in this paper also provide a certain theoretical feasibility and methodology base for further in-depth study of the quantitative relationships among shear stress, ATP, calcium signals and endothelial cellular function.