抑癌基因PTEN对人原代子宫内膜细胞凋亡及细胞周期的影响

Effect of tumor suppressor gene PTEN on human primary endometrial cell apoptosis and its cell cycle

目的:通过人原代子宫内膜细胞实验研究第10号染色体缺失的磷酸酶(phosphatase and tensin homolog deleted on chromosome ten,PTEN)对子宫内膜异位症发生、发展的作用和意义。方法 :利用慢病毒载体分别在人原代子宫内膜细胞中过表达和静默PTEN基因的表达;流式细胞仪检测慢病毒感染之后不同PTEN表达组的细胞周期及凋亡变化情况。结果:PTEN过表达组人原代子宫内膜细胞G0/G1期比例增加,与对照组比较差异具有统计学意义,G2/M期比例逐渐减少,细胞周期阻滞在G0/G1期;空白对照组、PTEN过表达组、PTEN静默组的细胞凋亡率分别是(11.70±0.03)%、(15.80±0.14)%、(5.33±0.08)%。结论:PTEN可显著增加人原代子宫内膜细胞凋亡,抑制细胞周期,对子宫内膜异位症的发生发展有一定抑制作用。

Objective：To study the role and significance of phosphatase and tensin homolog deleted on chromosome ten（PTEN）protein for the occurrence and development of endometriosis（EMs）in vitro experiments. Methods：Lentiviral vector was used to overexpress PTEN and silence PTEN expression in human primary endometrial cells; flow cytometry was used to detect the changes in cell cycle and apoptosis in the different PTEN expression groups after lentivirus infection. Results：Cell cycle analysis showed that after transfection of overexpressed lentivirus of PTEN gene,the proportion of G0 / G1 phase in human primary endometrial cells was increased,compared with the control group（P〈0.05）. The proportion of G2 / M phase was gradually decreased,and cell cycle was arrested in G0 / G1 phase; In the blank group,the overexpressed PTEN group,and the si PTEN group,cell apoptosis rates were（11.70± 0.03）%,（15.80 ± 0.14）%,and（5.33 ± 0.08）%,respectively. Conclusion：Overexpression of PTEN could significantly increase apoptosis rate and inhibit cell cycle of human primary endometrial cells. PTEN has a certain inhibiting effect on the occurrence and development of endometriosis.