结直肠癌特征性环状RNA筛选

Expression profile of circular RNA in colorectal cancer

目的:检测环状RNA(circular RNA,circ RNA)在结直肠癌组织及对应癌旁组织中的表达谱变化。方法 :应用高通量circ RNA芯片技术筛选6例人结直肠癌组织及对应癌旁组织中circ RNA表达谱差异,经过对原始数据进行预处理、归一化后,筛选出差异表达的circ RNA,并对其进行聚类分析。结果:结直肠癌组织与癌旁组织之间存在892种circ RNA的差异表达,其中在肿瘤组织中高表达的有412条,低表达的有480条(变化>2倍且P<0.05)。结论:结直肠癌组织与对应癌旁组织比较,circ RNA表达谱发生了显著变化,提示这些差异表达的circ RNA可能与结直肠癌的发生发展密切相关。

Objective：To analyze the expression profile variation of circular RNA（circ RNA） between normal colorectal tissues and colorectal cancer（CRC） tissues. Methods： Human circ RNA microarray was performed to detect the difference of circ RNA expression between 6 cases of CRC tissues and 6 cases of normal colorectal tissues. After pretreatment and homogenization of raw data,the circ RNAs with differental expression were screened out and then analyzed by hierarchical clustering and scatter volcano diagram.Results： Compared with normal colorectal tissues, there were 892 circ RNAs differentially expressed which have more than 2 times variation and significant difference（P〈0.05）. Among them, 412 increased and change′s fold more than 2 times,while 480 reduced and change′s fold more than 2 times. Conclusion： The circ RNA expression profile of CRC changed significantly in comparison with the normal colorectal tissues, the different circ RNAs may be associated with the development of CRC.