白藜芦醇通过抑制miR-27b表达促进白色脂肪细胞的棕色化功能

Resveratrol promotes the browning of white adipocytes via suppressing miR-27b expression

目的：探讨白藜芦醇对小鼠原代白色脂肪细胞棕色化功能的影响,研究白藜芦醇调控白色脂肪棕色化功能可能的分子机制。方法：从C57BL/5J小鼠的腹股沟处分离皮下前脂肪细胞,诱导分化后使用不同浓度的白藜芦醇（0~50μmol/L）刺激48 h,运用RT-PCR检测棕色标志因子基因及相关的mi R-27b表达情况。为了验证mi R-27b的作用,在细胞内过表达mi R-27b,同时予白藜芦醇刺激,运用RT-PCR和Western blot检测相关重要转录因子的基因及蛋白表达。结果：白藜芦醇促进了小鼠原代白色脂肪细胞棕色化功能基因的表达,抑制了原代白色脂肪细胞mi R-27b表达,并呈剂量效应。过表达mi R-27b显著抑制了白藜芦醇增强棕色化标志基因m RNA表达的作用。在蛋白水平上,过表达mi R-27b同样抑制了白藜芦醇增强其靶基因PRDM16和棕色标记基因UCP1蛋白表达的作用。结论：白藜芦醇通过抑制mi R-27b表达增强了白色脂肪细胞的棕色化功能,从而为临床有效改善机体能量代谢、防治代谢综合征提供了新策略。

Objective：To study the role of resveratrol in regulating the browning of mouse primary white adipocytes and find out the related molecular mechanisms. Methods：Primary subcutaneous preadipocytes were isolated from the groin of C57 BL / 5J mice and induced for differentiation. The cells were treated with different concentrations（0-50 μmol / L） of resveratrol to analyze the resveratrol′s effect on the browning of white adipocytes. Real-time PCR was performed to detect the m RNA expression of BAT-specific genes and mi R-27. mimic-mi R-27 b was transfected into primary cells to increase mi R-27 b expression,then,resveratrol（50 μmol / L）was performed to stimulate these cells. We used real-time PCR and Western blot to measure m RNA and protein levels of BAT-specific genes in these cells. Results：Resveratrol promoted the expression of BAT-specific genes and suppressed mi R-27 b expression in a concentration-dependent manner in mouse white adipocytes. Overexpression of mi R-27 b significantly inhibited the resveratrol action on the m RNA expression of BAT-specific genes. At the protein level,overexpression of mi R-27 b also suppressed the resveratrol action on the PRDM16 and UCP1 protein levels in mouse white adipocytes. Conclusion：Our data showed that resveratrol promoted the browning of mouse white adipocytes via suppressing mi R-27 b expression. Mi R-27 b might provide a potential therapeutic target for the treatment of obesity and metabolic syndrome.