隔药灸对急性胃黏膜损伤家兔穴区线粒体及Ⅰ型胶原蛋白的影响

Effect of Herbs-Partitioned Moxibustion on The Point Area Mitochondria and Collagen Type Ⅰof Acute Gastric Mucosal Injury Rabbits

目的:观察隔药灸对急性胃黏膜损伤家兔胃黏膜损伤指数、穴区局部线粒体结构、Ⅰ型胶原蛋白表达的影响,从胃黏膜修复及腧穴局部能量代谢角度探讨隔药灸对急性胃黏膜损伤的起效机制。方法:新西兰兔随机分为正常组、模型组、隔药灸组和西药组。在成功制备急性胃黏膜损伤模型的基础上,隔药灸组取"后三里穴"进行治疗,西药组以硫糖铝灌胃治疗。各组治疗干预结束后,留取胃黏膜和穴区局部组织,采用Guth法对各组的胃黏膜损伤指数进行评分,透射电镜观察后三里穴位局部组织线粒体形态结构,免疫组化法检测穴区Ⅰ型胶原蛋白的表达。结果:与正常组比较,模型组、西药组和隔药灸组家兔胃黏膜损伤指数均显著升高(均P<0.01);模型组穴区细胞线粒体空泡化,Ⅰ型胶原蛋白表达降低(P<0.01);隔药灸组仅有少数线粒体水肿;西药组线粒体数目少、出现肿胀,线粒体嵴不明显。与模型组比较,隔药灸组和西药组胃黏膜损伤指数显著下降(均P<0.01),Ⅰ型胶原蛋白表达升高(P<0.01或P<0.05)。隔药灸组胃黏膜损伤指数低于西药组(P<0.01),Ⅰ型胶原蛋白表达高于西药组,但差异无统计学意义(P>0.05)。结论:隔药灸能够改善急性胃黏膜损伤家兔"后三里"穴穴区线粒体形态、提高急性胃黏膜损伤家兔后三里穴局部组织的Ⅰ型胶原蛋白的表达,这可能是其修复胃黏膜损伤的起效途径之一。

Objective：To observe the effect of moxibustion on gastric mucosa injury index,the local structure of mitochondria and expression of type I collagen in acute gastric mucosal injury rabbits,and discuss the working mechanism of moxibustion on acute gastric mucosal injury from the perspective of gastric mucosal repair and energy metabolism of local acupoint.Methods：A group of New Zealand rabbits were randomly divided into normal group,model group,moxibuslion group and western medicine group.As acute gaslric mucosal injurv model was successfully prepared,moxibustion group used ST36（Housanli） treatment,western medicine group used sucralfate gavage treatment.After the intervention,gastric mucosa and acupoint local tissue was reserved,using Guth method to observe index of gastric mucosal injury and transmission electron microscope to view local tissue mitochondrial morphology of ST36（Housanli）.Also,immunohistochemistry was used to detect the expression of type Ⅰ collagen.Results：Compared with the normal group,gastric mucosal injur）＇ index of rabbits in model group,western medicine group and herbs-partitioned moxibustion group were significantly increased（P0.01）.Cells in acupoint of model group appeared mitochondrial vacuolation,and expression of type Ⅰ collagen was decreased（P〈0.01）.Besides,only a few mitochondria showed edema in moxibustion group and mitochondria decreased and mitochondrial cristae was not obvious in the western medicine group.Compared with the model group,gaslric mucosal injury index decreased significantly（P〈0.01）,while the expression of type Ⅰ collagen increased（P〈0.01 or P〈0.05） in moxibustion group and Western medicine group.Gastric mucosal injury index in moxibustion group was lower than that in western medicine group（P〈0.01）,thus type I collagen expression was higher than that in western medicine group,but the difference was not statistically significant（P〈0.05）.Conclusion：Herbs-partitioned moxibustion could improve mitochondrial morphology and increase the expression of collagen type I in acute gaslric mucosal injury rabbits,which may be an effective approach to repair gastric mucosal injury.