不同黑莓品种果实发育过程中生育酚合成规律研究

Study on the Synthesis of Vitamin E in Different Blackberry Varieties

以生产上表现优异的黑莓品种"宁植3号"和"Kiowa"为试验材料,采用高效液相色谱法(HPLC),在花后14d直至果实成熟过程中对黑莓种子及全果中生育酚含量和组分进行测定分析,以期为黑莓的进一步开发利用提供参考。结果表明:随着果实的发育,"宁植3号"和"Kiowa"种子中α-生育酚含量总体呈下降趋势,β+γ-和δ-生育酚含量呈上升趋势,生育酚总量不断上升;全果中α-生育酚含量亦呈现持续下降的趋势,β+γ-和δ-生育酚含量随着果实发育先升后降;全果和种子中不同组分生育酚的变化规律不一致,种子和全果中β+γ-生育酚积累量最多,α-生育酚含量次之,分析认为这可能是由不同的候选基因决定。

The synthesis and changes of vitamin E during blackberry fruit development were investigated in this study.The blackberry cultivars‘Ningzhi 3’and‘Kiowa’were used as the research materials,and the high-performance liquid chromatography(HPLC)was applied to determine the total amount of vitamin E and components in blackberry seeds and whole fruits at 14 days after flowering,and continued until the ripening of the fruit.The results showed that with the development of fruits,the contents ofα-tocopherol in the seeds of the two cultivars declined,while the contents ofβ+γ-andδ-tocopherol increased.The total vitamin E content increased continuously,which reached the maximum on the 35 th and 49 th days,and the contents were 36.47 mg·(100 g)-1 DW and 47.66 mg·(100 g)-1 DW,respectively.The content ofα-tocopherol in the whole fruits of the two cultivars also showed a continuous decrease trend,reaching minimum values on the 42 th and 49 th days,respectively,and the content ofβ+γ-andδ-tocopherol first increased and then decreased with fruits growth,theβ+γ-tocopherol content of the two cultivars reached the peak on the 35 thday,and theδ-tocopherol peaked on the 35 th and the 42 nd day respectively.In the early period of fruit development,the total content of vitamin E was maintained higher content in the whole fruit of both cultivars,the highest content was77.34 mg·(100 g)-1 DW and 72.76 mg·(100 g)-1 DW on the 35 th day,respectively,and then the content began to decrease after 35 days.In conclusion,the blackberry cultivars‘Ningzhi 3’and‘Kiowa’had higher content of vitamin E in the whole fruit and seeds,and the total content of vitamin E in the whole fruit was higher than that in the seeds.The changes of different tocopherols components in the whole fruit and seeds were different.The accumulation ofβ+γ-tocopherol was the highest in seeds and whole fruits,and the next wasα-tocopherol.It was considered that this changes may be determined by different candidate genes.