期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

HOXC6通过激活转化生长因子β信号通路增强肿瘤细胞活性 被引量：1

下载PDF
导出
摘要 目的通过转染肿瘤细胞,探讨同源异型盒C6(homeobox C6,HOXC6)在肿瘤进展中的作用机制。方法应用质粒转染、transwell、免疫荧光及Western blot检测肿瘤细胞中HOXC6表达对转化生长因子β(transforming growth factor-β,TGF-β)信号通路及肿瘤细胞活性的影响。结果过表达HOXC6的肝癌细胞Huh-7细胞迁移数明显高于未转染HOXC6组(P<0.01),磷酸化Smad2表达明显高于未转染HOXC6组(P<0.01),而Smad2/3表达明显少于未转染HOXC6组(P<0.01)。过表达HOXC6的肝癌细胞Huh-7Smad2在细胞核中表达强。结论 HOXC6过表达可激活TGF-β信号通路,增强肿瘤细胞活性。
作者 曹晓玮 阎锡新 刘姣姣 杨涛
机构地区 河北省石家庄市第一医院呼吸内科 河北医科大学第二医院呼吸内科 河北省石家庄市第一医院普外科
出处 《河北医科大学学报》 CAS 2015年第12期1420-1424,共5页 Journal of Hebei Medical University
基金 石家庄市科学技术研究与发展指导计划(141463243)
关键词 肿瘤细胞 培养的 基因 同源异型盒 转化生长因子Β
分类号 R394.26 [医药卫生—医学遗传学]
  • 相关文献

参考文献16

  • 1杨涛,孙轶飞,王立伟,张颖,白文彩.KIF14 过表达与肝细胞肝癌手术预后密切相关[J].河北医科大学学报,2014,35(3):263-265. 被引量：7
  • 2刘宏侠,孙玉满,谷守琦,李红民,杨俊泉,汪宏斌,徐艺东.促红细胞生成素及其受体在非小细胞肺癌中的表达及临床意义[J].临床荟萃,2011,26(15):1321-1325. 被引量：1
  • 3Morgan R, Boxall A, Harrington KJ, et al. Targeting HOX transcription factors in prostate cancer [J ]. BMC Urol, 2014, 14:17.
  • 4Ando H, Natsume A, Senga T, et al.Peptide-based inhibition of the HOXA9/PBX interaction retards the growth of human meningioma[J]. Cancer Chemother Pharmaeol, 2014,73 ( 1 ) : 53-60.
  • 5Daniels TR, Neacato Ⅱ, Rodriguez JA, et al. Disruption of HOX activity leads to cell death that can be enhanced by the interference of iron uptake in malignant B cells[J].Leukemia, 2010,24 (9) : 1555- 1565.
  • 6Errieo MC,Felieetti F,Bottero L,et al.The abrogation of the HOXB7/PBX2 complex induces apoptosis in melanoma through the miR-221&222-e-FOS pathway[J]. Int J Cancer, 2013,133 (4): 879- 892.
  • 7Li Z,Zhang Z, Li Y, et al. PBX3 is an important cofactor of HOXA9 in leukemogenesis[J]. Blood, 2013,121 (8) : 1422 - 1431.
  • 8Morgan R, Boxall A, Harrington KJ, et al. Targeting the HOX/PBX dimer in breast cancer[J]. Breast Cancer Res Treat,2012,136(2) :389-398.
  • 9Hussain l, Bhan A, Ansari KI, et al. Bisphenol-A induces expression of HOXC6, an estrogen-regulated homeobox- containing gene associated withbreast eancer [J]. Bioehim Biophys Aeta, 2015,1849(6) : 697- 708.
  • 10Harold AR,Hoogland AM,Smit F,et al.The role of HOXC6 in prostate cancer development [J]. Prostate, 2015,75 (16) : 1868-1876.

二级参考文献27

  • 1Jelkmann W. Molecular biology of erythropoietin[J]. Intern Med, 2004,43 (8) : 649-659.
  • 2Farrell F, Lee A. The erythropoietin receptor and its expression in tumor cells and other tissues[J]. Oncologist, 2004,9(Suppl 5) : 18-30.
  • 3Okazaki T,Ebihara S,Asada M,et al. Erythropoietin promotes the growth of tumors lacking its receptor and decreases survival of tumor-bearing mice by enhancing angiogenesis[J]. Neoplasia, 2008,10 (9) : 932-939.
  • 4PelekanouV,Kampa M,Kafousi M. et al. Erythropoietin and its receptor in breast cancer:correlation with steroid receptors and outcome[J]. Cancer Epidemiol Biomarkers Prev, 2007,16 (10):2016-2023.
  • 5Yasuda Y, Musha T, Tanaka H, et al. Inhibition of erythropoietin signalling destroys xenografts of ovarian and uterine cancers in nude miee[J]. Br J Cancer,2001,84(6) :836- 843.
  • 6Lai SY, Childs EE, Xi S, et al. Erythropoietin-mediated activation of JAK-STAT signaling contributes to cellular invasion in head and neck squamous cell carcinoma [J]. Oncogene, 2005,24(27) :4442-4449.
  • 7Saintigny P, Besse B, Callard P, et al. Erythropoietin and erythropoietin receptor coexpression is associated with poor survival in stage I non-small cell lung cancer[J]. Clin Cancer Res,2007,13(16) :4825-4831.
  • 8Jemal A, Siegel R, Xu J. Cancer statistics, 2010[J]. CA Cancer J Clin,2010,60(5) :277-300.
  • 9Lappin T. The cellular biology of erythropoietin receptors[J]. Oncologist, 2003,8 ( Suppl 1 ) : 15-18,.
  • 10Pasehos N,Lykissas MG,Beris AE. The role of erythropoietin as an inhibitor of tissue ischemia[J]. Int J Biol Sci,2008,4(3) 161-168.

共引文献6

同被引文献22

  • 1杨倩,马翔,李华驰,等.基因组学与蛋白质组学在结直肠癌研究中的进展[J].中国临床医师杂志,2013,7(3):131.
  • 2Inoue T,Umezawa A, Takenaka T, et al. The contribution of epithelial-mesenchymal .transition to renal fibrosis differs among kidney disease models[J].Kidney Int, 2015,87 (1) 233-238.
  • 3Ono H, Imoto I, Kozaki K, et al. SIX1 promotes epithelial- mesenchymal transition in colorectal cancer through ZEB1 activation [J].Oncogene,2012,31(47) :4923-34.
  • 4Ren YH,Liu K J, Wang M, et al. De-sumoylation of foxe2 by senp3 promotes the epithelial-mesenchymal transition in gastric cancer cell[J]. 0ncotarget,2014,5(16)t7093 -7104.
  • 5Lira SH,Becker TM, Chua W, et ah Circulating tumour cells and the epithelial mesenchymal transition in colorectal cancer [J]. Clin Pathol,2014,67(10) :848-853.
  • 6Liu Y, Chu A, Chakroun I, et ah Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation[J]. Nucleic Acids Res,2010,38(20):6857-6871.
  • 7Li Z, Tian T, Lv F, et al. Six1 promotes proliferation of pancreatic cancer ceils via upregulation of cyclin D1 expression[J]. PLoS One,2013,8(3) :357-361.
  • 8Wang CA, Jedlicka P, Patrick AN, et al. Sixl induces lym- phangiogenesis and metastasis via upregulation of VEGF-C in mouse models of breast caneer[J]. JClin Invest, 2012,122 (5) : 1895-1906.
  • 9Jin H, Cui M, Kong M, et al. Sineoculis homeobox homolog protin is assiciated with breast cancer progression and survival outcome[J]. Exp Mol Pathol, 2014,97 (2) : 247-252.
  • 10Ono H, Imoto I, Kozaki K, et al. SIX1 promotes epithelial- mesenchymal transition in colorectal cancer through ZEB1 activation[J]. Oncogene, 2012,31(47) : 4923-4934.

引证文献1

二级引证文献4

河北医科大学学报
2015年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部