摘要
目的用热药造热证细胞模型,研究＂热者寒之＂的基本治则对于细胞来说是否适用。方法分别用白胡椒50μg/m L、荜菝50μg/m L、高良姜100μg/m L、吴茱萸10μg/m L、肉桂20μg/m L进行造模,再以黄连、虎杖、白薇3味寒药分5~800μg/m L 8个浓度梯度分别进行逆转。结果黄连、虎杖和白薇对5种热药所造模型可以完全逆转,这与动物实验结果一致。结论 ＂热者寒之＂这一中医基本治则在细胞水平上是适用的,并将丰富细胞中药学的内容。
Objective To test whether the traditional therapeutic rule ＂to treat hot syndrome with cold drugs＂can apply to the hot syndrome cell model which was established with hot drugs. Methods Five hot herbal drugs white Peper（ 50 μg /m L）,Piper longum L.（ 50 μg /m L）,Alpinia officinarum Hance（ 100 μg /m L）,Evodia rutaecarpa（ Juss.）Benth（ 10 μg / m L） and Cinnamomum cassia presl.（ 20 μg / m L） were used to treat the cell to induce hot model. Then three cold herbal drugs Coptis Chinensis Franch,Povygonum cuspidatum sieb,et. Zucc and Cynanchum atratum Bge. 5- 800 μg / m L were used to reverse models. Result Three cold herbal drugs could completely cure the hot syndrome and the results were in accordance with that obtained on animal model. Conclusion The traditional therapeutic rule＂to treat hot symptom with cold drug＂can apply on cell model.
出处
《吉林中医药》
2015年第12期1255-1259,共5页
Jilin Journal of Chinese Medicine
基金
国家自然科学基金:以仿生合成策略设计合成钩藤中的单萜吲哚生物碱20872118)
陕西省自然科学基金资助项目:ATP
NADH与中药寒热药性的相关性研究(2014JM4125)
陕西省中医管理局基金资助项目(B-JC012)
陕西省教育厅专项基金资助项目:寒热中药对细胞能量代谢的机制研究(14JK1737)
