MTHFD2在肝细胞癌中表达的初步观察

The preliminary study on the function of methylenetetrahydrofolate dehydrogenase 2 in hepatocellular carcinoma

目的 观察亚甲基四氢叶酸脱氢酶2 （MTHFD2）在肝细胞癌（HCC）组织中的表达情况及其与临床病理参数之间的关系.方法 采用荧光定量PCR和免疫组织化学染色方法检测HCC患者癌及非癌组织中MTHFD2的表达情况,并分析MTHFD2表达水平与临床病理参数之间的关系.结果 HCC癌组织中的MTHFD2 mRNA水平高于其对应的非癌组织（31/47）,31例患者癌组织的MTHFD2 mRNA水平高于其对应的非癌组织,高表达率约为66％（P＜0.05）;MTHFD2蛋白在HCC癌组织中的表达（69.5％）明显高于非癌组织（33.9％）（P＜0.05）;癌组织中MTHFD2高表达与肿瘤转移、复发等临床病理参数相关（P＜0.05）,且MTHFD2高表达的患者术后无瘤生存期较低表达组明显缩短（P＜0.05）.结论 HCC癌组织中MTHFD2高表达与HCC转移、复发有密切关系,有可能成为一个新的预测HCC患者预后的生物标志物.

Objective To investigate the expression of methylenetetrahydrofolate dehydrogenase 2 （MTHFD2） in hepatocellular carcinoma （HCC） tissues and its correlation with clinical parameters.Method Fluorogenic quantitative-polymerase chain reaction （FQ-PCR） was used to measure the MTHFD2 mRNA expression.The MTHFD2 protein expression was detected by immunohistochemical staining in cancerous tissues and adjacent noncancerous counterparts.The relationship of MTHFD2 expression,clinicopathological parameters and the prognosis of hepatocellular carcinoma was subsequently analysed.Results The MTHFD2 mRNA expression in cancerous tissues was higher than that in adjacent noncancerous counterparts （31/47） （P 〈 0.05）.The positive rate of MTHFD2 protein in cancerous tissues was significantly higher than that in adjacent noncancerous counterparts （69.5% vs.33.9%） （P 〈 0.05）.MTHFD2 overexpression was found to correlate with clinical pathological parameters such as tumor metastasis,recurrence and poor prognosis （P 〈 0.05）.The patients with overexpressed MTHFD2 had shorter tumor-free survival time.Conclusions Overexpression of MTHFD2 in HCC may be a risk factor of tumor metastasis and recurrence.MTHFD2 could be a new biomarker for prognosis of HCC.