镇江地区男性患者人乳头瘤病毒基因型分析

Human papillomavirus genotypes in male patients attending the STD clinic in Zhenjiang area

目的:了解镇江地区男性人乳头瘤病毒(HPV)感染状况和基因分型情况。方法:运用PCR-反向点杂交法对245例男性患者进行HPV DNA分型检测。结果:245例受检者中检出107例阳性,HPV总感染率为43.67%,在所测的19种HPV亚型中检出18种。107例阳性者中,单纯低危型42例,占39.25%;单纯高危型41例,占38.32%;高低危混合感染24例,占22.43%。低危型常见亚型为HPV6和HPV11;高危型常见亚型为HPV16、52、58。单一感染、多重感染分别占53.27%(57/107)、46.73%(50/107),感染型别最多的1例为8个基因型。不同年龄段间HPV总感染率差异无统计学意义(χ2=7.999,P>0.05)。结论:HPV6、HPV11和HPV16是镇江地区男性最常见的感染,低危型以HPV6、11常见,高危型以HPV16、52、58常见。

Objective： To investigate the status of human papillomavirus（ HPV） infection and its genotypes in male patients in Zhenjiang area. Methods： Using PCR and reverse dot blot hybridization,we determined the genotypes of HPV DNA in 245 male patients at our Clinic of Dermatology and STD. Results： The total rate of HPV infection was 43. 67%（ 107 /245）,and 18 subtypes were detected. Among the 107 HPV-positive cases,low-risk,high-risk,and combined high- and low-risk infections accounted for39. 25%（ 42 /107）,38. 32%（ 41 /107）,and 22. 43%（ 24 /107）,respectively. The most notable low-risk HPV types were HPV6 and HPV11,and the most notable high-risk HPV types were HPV16,HPV52,and HPV58. The rates of single infection and multi-infection were 53. 27%（ 57 /107） and 46. 73%（ 50 /107）,respectively. One case had the most types,infected with 8 genotypes. No statistically significant differences were observed in the total rate of HPV infection among different age groups（ χ^2= 7. 999,P〉0. 05）.Conclusion： The dominant subtypes of HPV infection in male patients in Zhenjiang area were HPV6,HPV11,and HPV16. The most common subtypes were HPV6 and HPV11 in low-risk infection,and HPV16,HPV52,and HPV58 in high-risk infection.