偏头痛风湿夹瘀证动物模型的建立

Establishment of migraine rheumatism stasis syndrome animal model

目的:尝试建立偏头痛风湿夹瘀证动物模型。方法:使用人工气候箱风湿刺激加利血平化低5-HT伴局部脑血管痉挛协同诱导建立大鼠偏头痛风湿夹瘀证动物模型,通过观察动物一般生命体征(活动、体重、眼眵、毛发、进食、大便),挠头次数,图像采集、分析技术研究模型大鼠风湿夹瘀证生物表征(舌象RGB)的变化及动物凝血、五羟色胺等,观察模型动物是否符合风湿夹瘀症候特征。结果:利血平组与利血平加风湿造模组大鼠较对照组凝血时间明显缩短,痛阈降低,全血及脑内5-HT含量明显下降(P<0.01),利血平加风湿造模组大鼠眼眵增多,活动减少,进食减少,大便稀溏,舌象RGB值分析,对照组大鼠舌象淡红,利血平组舌象紫暗,利血平加风湿组舌质青灰,3组舌象RGB值有明显差异。结论:使用人工气候箱风湿刺激加利血平化低5-HT伴局部脑血管痉挛协同诱导可建立大鼠偏头痛风湿夹瘀证动物模型,但仍需进一步改进造模评价方法与流程。

Objective： To establish the migraine rheumatism stasis syndrome animal model. Method： The rat migraine rheumatism stasis syndrome animal model was established through rheumatism stimulation with manual climate box,5-HT reduction caused by reserpine and local cerebral vasospasm. General vital signs（ activity,weight,eye gum,hair,feeding,excrement）,head scratch frequency and image collection were observed to analyze the changes in biological signs of stasis syndrome（ tongue image RGB）,thrombin and serotonin of model rats. Result： The reserpine group and the reserpine plus rheumatism model group showed significant reduction in blood coagulation time,pain threshold and 5-HT content in blood and brain（ P 0. 01）; the reserpine plus rheumatism model group showed an increase in eye gum and decreases in activity,feeding,with thin sloppy stool. According to the tough RGB values,the control group showed light red toughs,the reserpine group showed dark purple toughs,the reserpine plus rheumatism model group showed gray toughs,with notable differences in tough RGB values in all three group. Conclusion： The rheumatism stimulation with manual climate box,5-HT reduction caused by reserpine and local cerebral vasospasm can be used to induce the migraine rheumatism stasis syndrome animal model,but its modeling assessment method and process shall be further improved.