微波辅助提取野坝子中总黄酮及其抑菌性能研究

Study on Microwave- assiste Extraction and Anti- microbial Activities of Total Flavones from the Leaves of Elsholtzia rugulosa

以云南野坝子的茎叶为原料,微波辅助法提取了野坝子中的总黄酮。在单因素实验基础上,使用正交试验法对提取工艺进行了优化,并与回流提取法进行了比较;采用滤纸片法对野坝子总黄酮粗提液对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌3种细菌进行了初步抑菌实验。结果表明:乙醇体积分数为70%,料液比为1∶30 g/m L,微波功率为350 W,微波时间为60 s时,提取效果最佳,总黄酮平均得率达2.98%,优于回流法;粗提液对枯草芽孢杆菌、大肠杆菌抑制效果较好,而对金黄色葡萄球菌无明显抑制作用。

Microwave -assiste technology was used to extract total flavonoids from Elsholtzia rugulo- sa. The influences on this assay strategy were carefully optimized by single factor test and orthogonal design test of L9 （ 34 ）, and then Compared with the Reflux extraction. Meanwhile, the bacteriostatic test of resul- ting extract was further emulated to escherichia coli, bacillus and saphyloccocus aureus. The resulted indica- ted that when the ethanol was 70% （wt % ） ,solid - liquid ratio was l：30g/mL, extracting time was 60s, and microwave power was 350W, the optimal amount of total flavonoids was 2. 81% better then the Reflux extraction. In addition, the extract has obvious antibacterial effect to escherichia coli and bacillus, but has no obvious effect to saphyloccocus aureus.