脱矿牙本质基质—富血小板血浆凝胶构建牙周骨组织工程支架的初步研究

Preliminary Study of Demineralized Dentin Matrix- Platelet-rich Plasma Gel Applying to Scaffold Material of Periodontal Bone Tissue Engineering

目的:评价自制脱矿牙本质基质(DDM)对富血小板血浆(PRP)作为生长因子缓释系统的影响。方法:二次离心法制备PRP凝胶,分为3组:A:PRP/DDM,B:PRP,C:全血。通过酶联免疫吸附法(ELISA)分别测定2周内单位体积各组凝胶释放的血小板衍化生长因子(PDGF)、转化生长因子-β(TGF-β)的水平。扫描电子显微镜下观察PRP/DDM凝胶的空间结构。将DDM植入SD大鼠股部肌肉陷窝内,1、3周后取材行组织学切片,观察有无毒性反应。结果:PRP/DDM凝胶较其两组持续释放PDGF和TGF-β的时间较长,组间差异有显著的统计学意义(P<0.05);经扫描电镜下观察及组织切片评估PRP与DDM结合紧密、有序;DDM具有很好的生物相容性。结论:DDM与PRP结合能够更好的促进生长因子的缓慢释放。

Objective：To evaluate the effects of demineralized dentin matrix（DDM）to the platelet-rich plasma（PRP）gel delivery system for growth factors.Methods：After double centrifugation for preparation of PRP,the concentrations of platelet-derived growth factor（PDGF）and transforming growth factor-β（TGF-β）in the whole blood gel（group C）,PRP gel（group B）,and PRP/DDM gel（group A）were measured in two weeks by enzyme-linked immunosorbent assay（ELISA）.The structure of prepared PRP/DDM gel was observed by scanning electron microscopy（SEM）.DDM was implanted into the pit of femoral muscles of SD rats and tissue sections were performed to study the toxicity reaction.Results：Compared with other two groups,PRP/DDM had a much longer time to continuously release the growth factors（P〈0.05）.Through SEM and tissue section,it was found that PRP and DDM combined with each other closely and orderly and DDM had a fine biocompatibility.Conclusion：The combination of PRP and DDM can better slower the release of growth factors.