微纳复合结构钛涂层的制备及其生物相容性研究

Study on the Fabrication and Biocompatibility of Hierarchical Titanium Surface

目的：在钛表面构建微纳复合结构涂层,并对其表面结构及生物相容性进行初步研究。方法：通过喷砂酸蚀、混合碱热处理的复合工艺,在钛表面构建新型微纳复合涂层,使用扫描电子显微镜（SEM）及X-射线能量分散分光计（EDS）对试件表征进行观察;使用CCK-8试剂盒检测该涂层的细胞毒性;进一步检测该涂层对于前成骨细胞（MC3T3-E1）粘附与增殖活性的影响。结果：钛表面微纳复合结构中,微米孔直径3-5μm,纳米孔直径100-200nm;新型涂层表面增加了钠元素和钙元素;体外细胞存活率实验表明该涂层细胞毒性0级;体外细胞粘附实验表明：第1、2h,细胞粘附活性在喷砂酸蚀处理组（MTi组）及混合碱处理组（NTi组）均明显强于单纯抛光组（STi组）,同时NTi组最强。体外细胞增殖实验表明：第1、4、7天MTi组的A值均小于STi组,具有统计学差异。NTi组的A值先是小于,后逐渐大于STi组。结论：相较于单独微米结构钛涂层,微纳复合结构钛涂层具有更接近于自然骨的分级结构以及更优良的生物活性。

Objective：To fabricate micro/nanostructures on titanium surface and analyze effects of hierarchical titanium surface on its biocompatibility.Methods：Mixed alkali treatment on the titanium surface was conducted after sandblast and acid etching,then scanning electronic microscope（SEM）and the X-ray energy dispersive spectrometer（EDS）were used to investigate microstructures of the titanium coatings.Survival rate of L-929 cells were detected by CCK-8kit.The adherent cells of each group were stained by DAPI,while the proliferation of preosteoblast of each group was investigated further.Results：On the hierarchical structures of titanium surface,the scale of micro pit was about 3-5μm,while the nanostructure was about 100-200 nm.Sodium and calcium were found on the new type surface.Cell toxicity was Class 0.Adhesion assay showed that the number of adherent cells both on MTi and NTi were bigger than that of STi,while cells on NTi were most at 1hour or 2hours.Proliferation test displayed that the proliferation activity on MTi was significantly lower than that on STi at day 1,4,7,while the proliferation activity on NTi was higher than that on STi at day 7.Conclusion：Hierarchical structure shows more biocompatible structure and better activity of adhesion and proliferation.