五倍子多糖相对分子质量和单糖组成的测定

Molecular Weight and Monosaccharide Components in Polysaccharides from Galla Chinensis

目的：研究五倍子多糖精细组分的相对分子质量、单糖组成。方法：五倍子多糖经提取纯化后得到GCP-1,GCP-2和GCP-3 3种精细组分。用高效凝胶渗透色谱（HPGPC）分析其相对分子质量;采用三氟乙酸（TFA）水解和1-苯基-3-甲基-5-吡唑啉酮（PMP）柱前衍生化的高效液相色谱（HPLC）分析其单糖组成。结果：HPGPC试验结果显示,GCP-1,GCP-2,GCP-3的相对分子质量分别为182,2.1×10~4,6×10~4;单糖组成试验表明,GCP-2和GCP-3的单糖组成类型基本一致,主要为半乳糖、半乳糖醛酸、葡萄糖醛酸等。结论：用HPGPC和PMP柱前衍生法测得五倍子多糖精细组分的相对分子质量和单糖组成,方法简便可行,可用于五倍子多糖的研究。

Objective： To determine the relative molecular weight and monosaccharide components in polysaccharides from Galla Chinensis（ GCP）. Method： Three fine compositions,GCP-1,GCP-2 and GCP-3 were obtained after extracting and purification of GCP. High performance gel permeation chromatography（ HPGPC） was used to analyze the average molecular weight; high performance liquid chromatography（ HPLC） of trifluoroacetic acid（ TFA） hydrolysis and 1-phenyl-3-methyl-5-pyrazolone（ PMP） pre-column derivatization was used to analyze its monosaccharide components. Result： According to HPGPC,the molecule weights were 182,2. 1 × 10~4 and 6 ×10~4, respectively for GCP-1, GCP-2 and GCP-3. The test of monosaccharide components showed that monosaccharide components of GCP-2 and GCP-3 were basically the same,mainly as galactose,galacturonic acid and glucuronic acid, etc. Conclusion： The two methods of HPGPC and PMP pre-column derivatization to determine the fine compositions of GCP are simple and convenient and could be applied to analysis of the molecular weight and monosaccharide composition in GCP.