加味四逆散对青少期应激模型大鼠行为学和HPA轴的影响

Effect of Modified Sini San on Behavior and HPA Axis of Adolescent Rats Model with Stress

目的:观察加味四逆散对青少期应激大鼠行为学、血浆促肾上腺皮质激素(ACTH)和血清皮质酮(CORT)浓度的影响。方法:32只SPF级雄性Wistar大鼠随机分为正常组,模型组,氟西汀组(0.01 g·kg^(-1)),加味四逆散组(16.9 g·kg^(-1)),采用慢性轻度不可预见性应激方法造模,造模同时ig给药28 d,每7 d称大鼠体重,检测糖水偏爱指数、旷场测试,采用放射免疫分析法检测大鼠血浆ACTH和血清CORT含量的改变。结果:与正常组比较,模型组大鼠出现体重增长缓慢、糖水偏爱指数明显降低,血浆ACTH和血清CORT含量明显升高(P<0.05),而旷场测试大鼠的活跃度、总路程和平均速度均有所升高,中央区活动时间有所延长,但差异无统计学意义;与模型组比较,氟西汀组和加味四逆散组糖水偏爱指数明显升高(P<0.05),血浆ACTH和血清CORT含量明显降低(P<0.05),氟西汀组体重在第28天明显增加(P<0.05)。结论:加味四逆散可以有效改善青少期应激大鼠行为学改变,下调其血浆ACTH和血清CORT含量,可能成为防治青少期应激损伤的有效方法。

Objective： To observe the effect of modified Sini San（ MSNS） on the behavior and concentration of plasma adrenocorticotropic hormone（ ACTH） and serum corticosterone（ CORT） in adolescent rats with stress. Method： The 32 male Wistar SPF rats were randomly divided into normal group,model group,fluoxetine group（ 0. 01 g ·kg-1）,MSNS group（ 16. 9 g ·kg-1）. The rats received 28 days chronic mild unexpected stimulation for modeling and intragastric administration. During the experiment,weighting,sucrose preference test and open field test were performed every 7 days. Plasma ACTH and CORT changes in rats were detected using radio immunoassay. Result： Compared with the normal group,the rats in model group showed a slow increase of body weight,decreased sucrose preference index,and significantly higher ACTH and CORT levels（ P 〈 0. 05）,while in the open field test,the activity,total distance and average speed in rats were increased and the central activity time had been prolonged,but with no statistical difference. Compared with the model group,the sucrose preference index of fluoxetine group and MSNS group was significantly higher（ P 〈 0. 05）,while ACTH and serum CORT levels were significantly lower（ P 〈 0. 05）,and the body weight of rats in fluoxetine group was significantly increased on 28 th day（ P 〈 0. 05）. Conclusion： MSNS can effectively improve behavior changes of stressed adolescent rats,lower plasma ACTH and serum CORT levels,so it may be an effective method to prevent and treat adolescent stress injuries.