18-β甘草次酸长期灌胃对大鼠肾脏的影响

Influence of Long-term Intragastric Administration with 18β-glycyrrhetinic Acid on Rat Kidney

目的:探讨18-β甘草次酸(glycyrrhetinic acid,GA)对大鼠肾脏的影响。方法:120只Wistar大鼠随机平均分成正常组及低、中、高剂量18-β甘草次酸组(25,50,100 mg·kg^(-1)),共4组,于甘草次酸ig干预后第6,14,22,30周及停药4周后心脏采血,检测血肌酐(SCr),尿素氮(BUN)含量,HE染色进行肾脏组织病理学观察。结果:随着干预进行直至停药4周时,低、中、高剂量甘草组SCr,BUN与正常组相比均无明显差异。血钠、氯离子均较正常组升高,但停药4周时血钠、氯离子较之前各时间点降低,并接近正常组。血钾离子均低于正常组,停药4周时血钾离子较之前各时间点升高并接近正常组。上述各指标同一时间点低、中、高剂量甘草组两两之间无统计差异。HE染色见各时间点肾脏病理形态与正常组相比未见异常改变。结论:18-β甘草次酸对大鼠血尿素氮、肌酐值及肾脏病理形态无明显影响。可引起血钠、氯离子轻度升高,血钾离子降低,停药一定时间后上述影响可消除。

Objective： To study on the influence of 18β-glycyrrhetinic acid（ GA） on rat kidney.Method： Totally Wistar rats were randomly and equally divided into four groups： normal group,low dose GA group,medium dose GA group,and high dose GA group（ 25,50,100 mg·kg-1）. Blood collection from cardiac puncture was conducted at the 6th,14 th,22nd,30 th week after GA intervention and at 4 weeks after GA withdrawal,to detect serum creatinine（ SCr）,and blood urea nitrogen（ BUN） levels. HE staining was done for kidney histopathological observation. Result： With the progress of intervention until 4 weeks after drug withdrawal,low-does,medium-does and high-does GA groups had no significant difference in SCr and BUN levels compared with the normal group. Compared with the normal group,serum sodium and chloride ions were increased.However,4 weeks after drug withdrawal,serum sodium and chloride ions in each group were decreased compared with the previous time points,getting close to the normal group. Serum potassium ions in these intervention groups were decreased as compared with the normal group. 4 weeks after drug withdrawal,serum potassium ions in each intervention group were increased,compared with the previous time points,getting close to the normal group.There was no statistical difference in above indicators at the same time points between the low-does,medium-dose and high-dose GA groups. Compared with the normal group,no abnormal change was found in kidney pathological morphology at each time point by HE staining method. Conclusion： 18β-glycyrrhetinic acid has no significant effect on rats’ blood urea nitrogen,creatinine value and kidney pathological morphology. It can lead to mild elevation of serum sodium and chloride ions and the decrease of serum potassium ions. The influence can be eliminated after certain time of drug withdrawal.