接种密度和蔗糖浓度对桔梗花药诱导率的影响

Influence of Inoculation Density and Sucrose Concentration on Induction Rate of Anther of Platycodon grandiflorum

以桔梗花药为外植体,分别在培养基中接种不同密度的花药和添加不同浓度的蔗糖,研究其对桔梗花药诱导率的影响。结果表明:MS+1.0mg/L 6-BA+0.5mg/L NAA为诱导培养基时,愈伤组织诱导率随着接种密度的增加而下降;MS+1.0mg/L 6-BA+0.2mg/L 2,4-D为诱导培养基时,愈伤组织诱导率随着接种密度的增加呈先升高后下降的趋势,密度为15枚/瓶时诱导率最高;且后者各处理的诱导率均明显高于前者。MS+1.0mg/L 6-BA+0.5mg/L NAA为诱导培养基,随着蔗糖浓度的增加,愈伤组织的诱导率呈先升高后下降的趋势,蔗糖浓度为60g/L时,愈伤组织诱导率最高。

Using the anther of Platycodon grandiflorum as explants,different density of anther were inoculated and different concentrations of sucrose were added respectively in the culture medium,and their effect on induction rate of anther was studied.The results showed that MS＋1.0mg/L 6-BA＋0.5mg/L NAA as induction medium,with the increase of density callus induction rate was decreased;MS＋1.0 mg/L 6-BA＋0.2mg/L BA＋2,4-D as induction medium,callus induction rate first increase and then decrease with the increase of density,when the density reach to 15,the induction rate was the highest;and the induction rate of the latter were significantly higher than that of the former induction rate.MS＋1.0 mg/L 6-BA＋0.5 mg/L NAA as induction medium,attaching different concentrations of sucrose,with the increase of sucrose concentration,callus induction rate showed a trend of rise after the fall of the first,while the concentration of sucrose reached to 60g/L,the induction rate was the highest.