近红外光谱法快速测定野菊花药材中水分及蒙花苷含量

Rapid Determination of Moisture and Linarin in Chrysanthemi Indici Flos by NIR Technique

目的应用近红外光谱技术建立一种野菊花药材中水分及有效成分含量的快速分析方法。方法采集45批野菊花药材的近红外光谱,分别用烘干法和HPLC测得药材中水分及蒙花苷含量值作为参考,运用偏最小二乘法(PLS)建立野菊花药材水分及蒙花苷的定量模型,并用未知样品进行验证。结果野菊花药材水分和有效成分蒙花苷含量PLS模型得到的NIR预测值与实测值有较好的相关性,模型的交互验证误差均方根(RMSECV)分别为0.568和0.282;相关系数R2为0.976 1和0.955 1;RESP均控制在10%以内,完全符合中药生产过程实时分析的精度要求。结论研究表明近红外光谱技术能够快速测定野菊花药材水分和蒙花苷含量,有助于药材入库的快速检验,节省人力物力。

OBJECTIVE To establish a rapid analytical method for determining moisture and linarin content in Chrysanthemi Indici Flos by near-infrared reflectance（NIR） technique. METHODS The NIR spectroscopy data of 45 batches of samples were collected, the data of moisture and linarin content as reference were obtained by drying method and HPLC, respectively. The quantitative model for both was established by partial least squares（PLS） and verified by unknown samples. RESULTS There is a good correlation between the predicted NIR values and the actual values. The root-mean-square error of cross-validation（RMSECV） of the quantitative calibration model of moisture and linarin was 0.568 and 0.282, respectively. The correlation coefficients R2 was 0.976 1 and 0.955 1, respectively, and the RESP was both controlled within 10%, which were fully meeting the accuracy requirements of real time process analysis of TCM manufacturing. CONCLUSION NIR can achieve rapid determination of moisture and linarin in Chrysanthemi Indici Flos, which can determinate medicinal materials in storage rapidly and save manpower and material resources.