蔬菜产亚硝酸盐有害微生物快速检测

Rapid Detection of Spoilage Organisms Nitrite Ducing from Vegetables

现代研究表明,蔬菜中的亚硝酸盐是比农药危害更大的一种成分。蔬菜表面有害微生物通过产硝酸还原酶将硝酸盐还原成亚硝酸盐。随着基因组测序的快速发展,几乎所有微生物的基因组已经完全测序,使用保守区法,基于现有硝酸还原酶基因序列的比对而找到保守区,通过保守区设计简并引物。通过对硝酸还原酶基因nap A和nar G序列的比对,设计出两套简并引物,利用简并引物从基因组中扩增得到硝酸还原酶基因的片段。建立了一个快速、有效的不需要微生物培养的分子生物学方法用于分析检测蔬菜表面主要有害微生物的硝酸还原酶基因。

Modern research had shown that nitrite was more harmful than pesticides as an ingredient in vegetables. Microorganisms on the surface of vegetables reduced nitrate into nitrite by nitrate reductase. With the rapid development of genome sequencing, almost all microbial genomes had been completely sequenced. Use the method of conservative region, based on existing nitrate reductase gene sequence comparison, to f ind the conservative district, through the conservative region design degenerate primers. Based on the sequence alignments of napA and narG gene, two sets of primers were designed. Develop a rapid and eff icient culture independent molecular method to explore the nitrate reductase genes in bacteria on the surface of vegetables.