囊谦黑青稞花青素含量、种类及其抗氧化活性分析

Content, Species and Antioxidant Activity of Anthocyanin from Nangqian Black Hulless Barley

采用pH示差法测定了囊谦黑青稞中花青素含量,用HPLC法分析了囊谦黑青稞花青素种类,并通过DPPH法、ABTS法和FRAP法评价了花青素的抗氧化活性。结果表明,囊谦黑青稞花青素含量为8.27 mg/100 g,主要由8种花青素成分组成,对DPPH、ABTS自由基清除能力和总抗氧化能力显著优于VC,分别是VC的33.76,8.03和41.44倍,具有强抗氧化活性。

The content of anthocyanin from Nangqian black hulless barley was determined by pH-differential spectrophotometry and anthocyanin species was analyzed by HPLC method. Antioxidant activities of black hulless barley anthocyanin were evaluated by DPPH scavenging assay, ABTS scavenging assay and total antioxidant capacity assay. The results showed that anthocyanin content in black hulless barley was 8.27 mg/100 g and it contained 8 species. DPPH, ABTS free radical scavenging activities and total antioxidant capacity of black hulless barley anthocyanin were higher than that of VC and revealed 33.76, 8.03 and 41.44-fold enhancement when compared with VC. Black hulless barley anthocyanin possessed high antioxidant activity, which can be developed as natural antioxidants.