不同放置时间对融化后新鲜冰冻血浆中凝血因子的影响

Effect of different time on coagulation factors in fresh frozen plasma after thawing

目的探讨不同放置时间对融化后新鲜冰冻血浆中凝血因子的影响。方法选择本院血站经ACD-B抗凝全血分离制备而成的新鲜冰冻血浆30份,37℃水浴融化,于融化后0、6、12、24h测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白(FIB)、凝血酶时间(TT)、凝血因子FⅦ、FⅧ、FⅨ活性水平。结果新鲜冷冻血浆融化后,PT、APTT、FIB、TT在24h内不同时间点测定结果 ,差异无统计学意义(P>0.05)。凝血因子FⅦ及FⅧ随时间的改变而有明显的降低,随着时间的推移衰减,与抽血即刻比较差异有统计学意义(P<0.05)。而FⅨ仅在12h的时候有一过性的下降,24h后又恢复。结论新鲜冰冻血浆在融化后应该立即输注,以保证凝血因子生物学活性,进一步保证治疗的效果。

Objective To investigate the effect of different time on coagulation factors in fresh frozen plasma after thawing. Methods The medicine blood by ACD B anticoagulated whole blood separation, preparation and fresh frozen plasma30,37℃ water bath melting and melt after 0,6,12,24 h determination of prothrombin time（PT）,activated partial thromboplastin time（APTT）,fibrinogen（FIB）,thrombin time（TT）,coagulation factor F Ⅶ、F Ⅷ、F Ⅸ activity level. Results After the fresh frozen plasma,the PT,APTT,FIB,TT at different time points of the test results were not statistically different in 24 hours（P〉0.05）.Coagulation factor F Ⅶ and F Ⅷ changed with time and significantly decreased with the passage of time attenuation and blood immediate difference was statistically significant（P〈0.05）.FIX only when 12 hours had decreased,again after 24 hours. Conclusion Fresh frozen plasma should be infused immediately after thawing,so as to guarantee the biological activity of coagulation factor and guarantee the effect of treatment.