摘要
一项血清酶测定结果的可溯源性研究发现,碱性磷酸酶(ALP)在多种生化分析仪上的测定结果与校正物的定值相差悬殊,并且不同来源的ALP试剂盒的测定结果间也存在高低不一的系统误差。本文分析了系统误差的产生原因,从理论和实验两个方面排除了生化分析仪的原因,指出影响ALP测定结果的关键因素是缓冲液的种类,并以此为例说明在进行血清酶活性测定结果的溯源、校正和室间比对时,必须考虑构成检测系统的各个要素,包括试剂盒的性能质量和生化分析仪的参数设置。
A examination to the traceability of alkaline phosphatase（ALP） activity discovered that laboratory ALP activity measurement results existed big differences from the fixed value of enzyme reference material, and higher or lower system errors existed between ALP kits from different sources. This review analyzed the reason of these system errors, indicated that the key influencing factor was buffer system type in ALP assay. Taking ALP as an example, it indicated that traceability, calibration and external laboratory quality assessment of serum enzymes activity measurement must consider the every conditions of analysis system, including performance quality of reagent kit and parameter settings of analyzer.
出处
《中国医疗设备》
2015年第12期49-52,共4页
China Medical Devices
关键词
碱性磷酸酶
生化分析仪
酶活性测定
结果溯源
室间质评
alkaline phosphatase
biochemical analyzer
determination of the enzyme activity
result traceability
room quality assessment
