青南藏区多房棘球绦虫系统发育学分析

PRELIMINARY PHYLOGENETIC ANALYSIS OF Echinococcus multilocular IN QINGHAI SOUTHERN PLATEAU INFERRED BY CYTOCHROME OXIDASE SUBUNIT I GENE SEQUENCE

目的对青南藏区流行的多房棘球绦虫基因多态性及系统发育进行初步分析。方法利用线粒体DNA细胞色素氧化酶亚单位Ⅰ(COXⅠ)行分子标记,以COXⅠ为基因marker设计引物扩增COXⅠ部分基因序列,将得到的11条碱基序列通过Clustal X软件进行多重序列分析比对,与13条来自Gen Bank的棘球绦虫COXⅠ序列共同构建贝叶斯进化树。结果用于分析的标本序列与来源于亚洲的中国(四川,内蒙古)、哈萨克斯坦和欧洲的法国、斯洛伐克的多房棘球绦虫型聚于一枝,位于发育树的冠部,并在这一枝内部形成梳齿状分支。结论流行于青南藏区的多房棘球蚴标本存在基因多态性,其系统发育复杂。

Objective To analysis the phylogeny and genetic polymorphism of Echinococcus multilocular in Qinghai southern plateau. Methods We designed and synthesized the primers of COX Ⅰ gene,and used PCR to cloned 11 sequences from hepatic sample which infected by Echinococcus multilocular. The sequences were aligned using Clustal X,and then,Bayesian analyses were performed with 13 sequences of COX Ⅰ gene from the Gen Bank.Results In the phylogenetic tree,all of Echinococcus multilocular isolates belongs to Echinococcus multilocular which originate from different places,such as China（ Sichuan,Inner Mongolia）,Kazakhstan of Asia,France and Slovakia of Europe,they located on the phylogenetic tree crown,and form a comb shaped branch in this branch of internal.Conclusion There are many gene polymorphism concerning Echinococcus multilocularis specimens which specially popular in the southern regions of Qinghai province.