奶牛布鲁氏菌病的病原分离与鉴定

Pathogen Isolation and Identification of Cow Brucellosis

本试验对疑似布鲁氏菌病的样本进行了病原分离,采用血清学试验、染色镜检、PCR和基因克隆测序对分离菌株进行了鉴定。结果表明,4株分离菌株均为布鲁氏菌属细菌,与布鲁氏菌参考菌株的核苷酸同源性为99.52%~100%。采用AMOS PCR对4株分离菌株进行种特异性鉴定,结果显示,1株为猪种布鲁氏菌,3株为牛种布鲁氏菌。

In order to provide technical reference for laboratory diagnosis of cow brucellosis, the bacteria were isolated from suspected brucellosis cases. The isolated bacteria were identificated by serological method,staining and microscopy,PCR and gene cloning and sequencing method. The results showed that the four isolated bacteria were Brucella,the homology of nucleotide between four isolated bacteria and two reference strains were 99.52%~100%. The species specificity of four isolated bacteria were identificated by AMOS PCR method,the results showed that a bacterial strain was B.suis and three strains of bacteria were B.abortus.