辣椒碱分子印迹聚合物的合成研究

Study on synthesis of capsaicin molecularly imprinted polymers

为得到吸附性能优异且易于应用的辣椒碱分子印迹聚合物,对聚合体系及单体和交联剂的比例等进行了实验研究。通过比较聚合物形态和对模板分子的吸附能力大小,最终选择以α-甲基丙烯酸（MAA）为功能单体,以乙二醇二甲基丙烯酸酯（EDMA）为交联剂（MAA与EDMA的摩尔比为1∶4）,在以AIBN为引发剂的条件下,沉淀聚合法合成辣椒碱分子印迹聚合物。合成得到的聚合物MIP1经电镜扫描知其为形态均一的微球,粒径在500~1 000nm。平衡吸附实验表明,聚合物MIP1对辣椒碱的吸附能力,在辣椒碱初始质量浓度为0.03~0.8mg/mL范围内,均明显高于其空白印迹聚合物NIP1,说明MIP1对辣椒碱的吸附作用为特异性吸附,可进一步应用于固相萃取等样品预处理中。

To obtain capsaicin molecularly imprinted polymer with excellent adsorption which can be widely used,the reaction conditions such as polymerization systems and the molar ration of function monomer to cross-linker have been optimized.By comparing the morphologies and adsorption capabilities for capsaicin,a new method using MAA as functional monomer and EDMA as cross-linker（molar ratio of MAA to EDMA is 1∶4）and AIBN as initiator by precipitation polymerization have been established.Scanning electron microscopy（SEM）showed that the molecularly imprinted polymer microspheres（MIP1）synthesized were uniformly globular and the particle size was in the range of 500 nm to 1 000 nm.The equilibrium adsorptionexperiments showed that the adsorption efficiency of MIP1 had favorable higher binding ability for capsaicin compared with NIP1 in the capsaicin initial concentration range of 0.03to0.8mg/mL.The results showed that MIP1 possesses the specific adsorptivity toward the template molecule,which can be further used in the solid phase extraction for separation and enrichment.