HPLC法测定延丹胶囊中原儿茶醛、芍药苷和橙皮苷的含量

HPLC Method in Detection of Contents of Protocatechuic Aldehyde,Paeoniflorin and Hesperidins in Yandan Capsules

目的建立控制延丹胶囊质量的方法。方法采用高效液相色谱（high performance liquid chromatography,HPLC）法同时测定延丹胶囊中原儿茶醛、芍药苷和橙皮苷的含量,Waters CAPCELL PAK C18色谱柱（150 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸,检验波长230nm,柱温25℃。结果原儿茶醛、芍药苷和橙皮苷分别在0.007728～0.4508μg（相关系数r=1.0000）、0.06462～4.308μg（r=1.0000）和0.06984～3.492μg（r=0.9999）呈良好线性关系;平均回收率（n=6）为99.00%[相对标准偏差（RSD）为1.10%]、98.92%（RSD为1.10%）,98.65%（RSD为1.10%）。结论该方法简便、准确、重复性好,可作为延丹胶囊质量控制方法。

Objective To establish a method to control the quality of Yandan capsules. Methods The highperformance liquid chromatography（ HPLC） method and a CAPCELL PAK C18（ 150 mm × 4. 6 mm,5 μm） chromatographic column were used; acetonitrile and 0. 1 % phosphoric acid solution were used as mobile phase in gradient mode.The wavelength was 30 nm with 25℃ column temperature. Results The Protocatechuic Aldehyde,Paeoniflorin and Hesperidin showed good linear correlation in the ranges of 0. 007728-0. 4508 μg [correlation coefficient（ r） = 1. 0000],0. 06462-4. 308 μg（ r = 1. 0000） and 0. 06984-3. 492 μg（ r = 0. 9999） respectively,and the average recovery rates were 99. 00 % [the relative standard deviation（ RSD） ∶ 1. 10 % ],98. 92 %（ RSD∶ 1. 10 %） and 98. 65%（ RSD∶1. 10 %） respectively. Conclusion The method is simple and feasible with good repeatability,and it can be used for the quality control of Yandan capsules.