吸烟对慢性牙周炎患者龈沟液及牙龈组织中β防御素2、3的影响

The impact of smoking on human beta defensin 2,3 in gingival crevicular fluid and gingival tissue of patients with chronic periodontitis

目的 :探讨吸烟对慢性牙周炎患者龈沟液(gingival crevicular fluid,GCF)及牙龈组织中β防御素(human beta def-ensin,hBD)2、3表达的影响。方法 :将研究对象分为吸烟慢性牙周炎组和非吸烟慢性牙周炎组。采用酶联免疫吸附法(Elisa)检测hBD2、3的浓度;反转录多聚合酶链反应(RT-PCR)检测hBD2、3m RNA的表达,并做半定量分析。相关数据采用SPSS 17.0软件包进行统计学分析。结果:在GCF中,hBD2、3在吸烟组的水平表达均低于非吸烟组;hBD2、3在2组牙龈组织样本中均有m RNA表达,其中吸烟组较非吸烟组m RNA表达水平减弱。结论 :吸烟可使GCF及牙龈组织中hBD2、3的表达发生改变,提示吸烟可对牙周宿主免疫防御系统产生消极影响。

PURPOSE： To study the impact of smoking on human beta defensin 2,3 expression in gingival crevicular fluid and gingival tissue of patients with chronic periodontitis.METHODS： The study subjects were divided into chronic periodontitis with smoking and without smoking.Enzyme linked immunosorbent assay（ELISA） was used to detect the concentration of hBD2,3.RT-PCR was used to detect the m RNA expression of hBD2,3.SPSS17.0 software package was used for statistical analysis.RESULTS： In GCF,the expression level of hBD2,3 in the smoking group was significantly lower than that in the non-smoking group.The m RNA expression level of hBD2,3 in the smoking group was weakened compared with that in the non-smoking group.CONCLUSIONS： Smoking can change the expression of hBD2,3 in both GCF and gingivae,which indicates that smoking may have a negative effect on the immune defense system of the periodontal host.