生姜粗多糖的提取及对脑缺血再灌注损伤大鼠的保护作用

Study on Extraction of Ginger Polysaccharide and Its Protecting Effect on Cerebral Ischemia- Reperfusion Injury

目的:探讨生姜粗多糖的超声提取的最佳条件,及其对大鼠脑缺血再灌注损伤的保护作用。方法:以生姜为原料选择最佳提取条件通过超声方法提取生姜粗多糖,并测定其含量;采用线栓法建立大鼠大脑动脉脑缺血再灌注损伤模型,通过生姜粗多糖对大鼠行为障碍评分的影响,对大鼠血清SOD和MDA的影响,对大鼠脑组织中SOD、MDA及NO含量的影响以及对大鼠脑含水量的影响来研究生姜多糖对大鼠脑缺血再灌注损伤的保护作用。结果:经提取后得生姜粗多糖5.56 g,通过计算得多糖含量为28.25%,与模型组比较,生姜粗多糖高、低剂量组及舒血宁组能明显改善大鼠的行为障碍(P<0.01,P<0.05),生姜粗多糖高、低剂量组及舒血宁组能明显升高大鼠血清中SOD活性(P<0.01)和明显降低大鼠血清中MDA含量(P<0.01,P<0.05),生姜粗多糖高、低剂量组及舒血宁组可升高大鼠脑组织中SOD活性(P<0.05),降低MDA和NO(P<0.01)的含量,生姜粗多糖高、低剂量组及舒血宁组可显著降低脑含水量(P<0.01)。结论:生姜粗多糖对大鼠脑缺血再灌注损伤具有保护作用。

Objective： To explore the best condition of ultrasonic extraction of the ginger polysaccharides extract and its certain protection effect of cerebral ischemia- reperfusion injury in rats. Methods： With the ginger as raw material,we chose the best extraction conditions by ultrasound method and determined its content. After treating the cerebral ischemia- reperfusion injury model rats,we collected the blood of all rats to measure the activity of superoxide dismutase（ SOD） and the contents of malondialdehyde（ MDA） in all groups were tested. The content of NO in all groups were tested and the effect of the brain water content was researched to observe the protection effect of cerebral ischemia- reperfusion injury in rats. Results： After ultrasonic extraction,the ginger polysaccharides basic flavonoids was 5. 56 g and through the regression equation the flavonoids content was 28. 25%. The ginger polysaccharide high and low dose groups and Shuxuening could significantly increase serum SOD activity（ P〈0.01） and significantly decrease MDA content（ P〈0.01,P〈0.05）. The ginger polysaccharide high and low dose groups and Shuxuening could significantly reduced brain water content（ P〈0.01）. Conclusion： Ginger polysaccharides has a certain protection effect of cerebral ischemia- reperfusion injury in rats.