摘要
为研究高山离子芥Cb PLDα、Cb PLDβ转基因烟草对低温胁迫的生理响应,采用4、0℃低温胁迫,揭示了Cb PLDα、Cb PLDβ基因在植物抵抗低温中的作用。结果表明:随着4℃胁迫时间的延长,野生型烟草和转基因烟草的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性以及抗坏血酸过氧化物酶(APX)活性均呈增加趋势,过氧化物酶(POD)和过氧化氢酶(CAT)活性则呈先上升后下降趋势。0℃胁迫条件下,野生型烟草和转基因烟草的丙二醛(MDA)含量和过氧化氢酶(CAT)活性以及抗坏血酸过氧化物酶(APX)均先上升后下降,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性呈增加趋势。Cb PLDα、Cb PLDβ转基因活性烟草的MDA含量显著低于野生型烟草植株,而抗氧化酶活性显著高于野生型烟草对照,且转基因烟草对0℃低温胁迫的响应更积极敏感。Cb PLDα、Cb PLDβ基因的导入提高了烟草幼苗的抗低温能力。
To study the physiological response of Chorispora bungeana Cb PLDα and Cb PLDβ transgenictobacco to low temperature, the authors used 4℃ and 0℃ temperature stresses to reveal the role of Cb PLDαand Cb PLDβ in low temperature resistance in plants. The results showed that the content of MDA, the activityof SOD and APX increased under 4℃ treatment, while the activity of POD and CAT decreased with the time.However, under 0℃ treatment, the content of MDA, the activity of CAT and APX decreased with the time,while the activity of SOD and POD increased. Under the cold stress, the activities of POD, SOD and CAT intransgenic tobacco plants were obviously higher than those in the non-transgenic tobacco plants. The contentsof MDA in transgenic tobacco plants were obviously lower than those in the non-transgenic tobacco plants. Theresults showed that the over expressing of Cb PLDα and Cb PLDβ gene improved the cold resistance of tobacco.
出处
《中国农学通报》
2015年第34期209-215,共7页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"高山离子芥细胞磷脂酶D在植物抗寒生理生态中的调控机制研究"(31160087)
国家自然科学基金"磷脂酶响应二萜类化感物质作用的分子及生理调控机制研究"(31360061)
