环境诱因引起大面积死亡背景下底播增殖仿刺参生物体优势菌分析

Dominant Bacteria in Sea Cucumber Apostichopus japonicus Released in Area Where Massive Death Was Caused by Environmental Triggers

为获知环境诱因引起底播增殖仿刺参大面积死亡背景下仿刺参生物体的优势菌,采集莱州金城和牟平两个地区事发海域的仿刺参样品,利用平板分离法筛选得到优势菌13株,经细菌16SrDNA通用引物扩增后,PCR产物进行回收、克隆测序、序列分析、RDP比对,结果显示:13株优势菌归为5个属,分别为副球菌属、动性球菌属、射光杆菌属、弧菌属、琼胶菌属,其中弧菌属细菌占比为53.8%。优势菌类型与仿刺参自身生理功能及养殖环境状况密切相关,两个取样地区优势菌类型有所差别,其中莱州海域弧菌属细菌比例高于牟平海域样品,养殖环境弧菌状况的差别是参体上的弧菌差别的主要影响因素。

The dominant bacteria were isolated from sea cucumbers released in Jincheng area in Laizhou and Muping area where the massive death of the sea cucumber caused by environmental factors had been oc-curred by plate-separation method ,and identified by 16S rDNA universal primer of bacteria ,by cloning , sequencing ,and RDP sequence alignment successively .The results showed that the 13 dominant bacterial strains were within 5 genera ,Paracoccus ,Planococcus ,Lucibacterium ,Vibrio and Agarivorans ,Vibrio bacteria accounting for 53 .8% .Dominant bacteria were correlated with the physiological function of sea cucumber and the aquaculture environment conditions .There were different dominant bacterial species in the samples collected from different areas ,more percentage of Vibrio in Laizhou area than that in Muping area ,w hich may be involved in the difference in V ibrio status in the culture environment .