嗜水气单胞菌感染对黄鳝hepcidin抗菌肽基因表达的影响

Effects of Aeromonas hydrophila on Hepcidin Gene Expression of Field Eel Monopterus albus

为了研究嗜水气单胞菌对黄鳝hepcidin抗菌肽基因表达的影响,用不同密度嗜水气单胞菌感染健康黄鳝后,采用荧光定量PCR方法分别检测染菌黄鳝肾脏hepcidin抗菌肽基因在感染后24h、48h、72h的表达量。试验结果表明,hepcidin基因在健康黄鳝中就有表达,腹腔注射嗜水气单胞菌后,该基因的表达量明显升高。随着处理时间的延长,处理菌密度较低组(0.5×108 cfu/mL)和较高组(2×108 cfu/mL和4×108 cfu/mL)黄鳝抗菌肽hepcidin基因的表达量都逐渐降低,只有菌密度为1×108cfu/mL时该基因的表达量是随着处理时间的延长而逐渐升高,但升高的幅度逐渐减小。密度为1×108 cfu/mL的嗜水气单胞菌能诱导黄鳝hepcidin抗菌肽基因表达量逐渐增加。

The expression level of hepcidin gene was monitored in field eel Monopterus albus artificially in-fected by different concentration of Aeromonas hydrophila 24 ,48 ,and 72 hours of infection by the methods of FQ-PCR .The results showed that hepcidin transcripts were detected in kidney of healthy field eel . When the field eel was challenged with A .hydrophila injection ,the hepcidin expression quantity was in-creased significantly .The hepcidin transcripts decreased as time prolonged in mostly tested groups（0 .5 × 108 cfu/mL ,2 × 108 cfu/mL and 4 × 108 cfu/mL） ,but increased in the group treated with A .hydrophila at concentration of 1 × 108cfu/mL .The finding showed that the hepcidin expression quantity was increased in field eel with A .hydrophila injection at concentration of 1 × 108cfu/mL .