SPF动物实验室Wistar大鼠正常生理生化参考值数据库的建立

Normal physiological and biochemical reference value range of Wistar rats in SPF animal lab

目的建立一套完整的毒理学动物背景数据库。方法收集整理2012年某机构SPF级动物实验室30 d喂养实验中的960只Wistar大鼠对照组毒理学基础指标的检测结果,计算其均值和正常参考值范围,分析各项指标间差异与联系。结果(1)雌雄体重差异具有统计学意义(P<0.05),体重与摄食量、脏器重量均间呈线性相关;(2)雌雄鼠间血常规差别无统计学差异(P>0.05),血生化检测部分指标差异具有统计学意义(P<0.05)。结论研究建立了一套较完整的SPF级Wistar大鼠背景数据库,通过与该机构不同时期数据进行比较,结果表明大部分指标的标准差较前减小,数据间离散程度有所降低。

Objective To set up a complete animals＇ database of toxicology background. Methods Collecting the result of the 960 Wistar rats in control group, and the rats were based on 30 day-feeding experiments which was performed in a SPF animal lab. Analyzing the mean and normal reference range as well as the connection between the indexes. Results（ 1） Weight difference between male and female rats were of significant difference（ P 0. 05）. The relationship between body weight and food intake,as well as viscera weight,presented a linear correlation.（ 2） Routine blood difference showed no statistical difference between male and female rats（ P 0. 05）,while some blood biochemical detection showed significant difference（ P 0. 05）. Conclusion This research set up a relatively complete background database of Wistar rats at SPF grade. Comparing with the data of rats during different periods in the center,the standard deviations and the discrete degree of most indicators were reduced.