骨髓间充质干细胞静脉移植对脊髓损伤大鼠生长相关蛋白-43表达的影响

Effects of Bone Marrow Mesenchymal Stem Cells Transplantation on Expression of Growth Associated Protein-43 in Rats after Spinal Cord Injury

目的观察骨髓间充质干细胞(BMSCs)静脉移植对脊髓损伤(SCI)大鼠生长相关蛋白(GAP)-43基因及蛋白表达的影响,探讨BMSCs静脉移植治疗SCI的作用机制。方法采用SCI打击器建立大鼠T11脊髓撞击损伤模型,造模成功后,将大鼠随机分为对照组(n=18)和观察组(n=18)。SCI后1周,对照组大鼠自尾静脉注射无血清的DMEM/F12培养液0.1 ml,观察组大鼠自尾静脉注射BMSCs悬液0.1 ml。SCI后1、2、4、8周,两组大鼠均进行BBB后肢运动功能评分;SCI后2、4、8周,采用RT-PCR检测GAP-43 m RNA的表达,采用免疫组化染色检测GAP-43蛋白的表达。结果与对照组比较,观察组SCI后2、4、8周BBB评分显著升高(P<0.001),GAP-43阳性表达显著增强(P<0.001),GAP-43 m RNA的相对表达量升高(P<0.05)。结论 BMSCs移植能增强SCI大鼠GAP-43在基因及蛋白水平的表达,从而促进SCI的再生修复。

Objective To observe the effect of bone marrow mesenchymal stem cells（BMSCs） transplantation on the expression of growth associated protein-43（GAP-43） in rats after spinal cord injury（SCI）. Methods The SCI models were made by impacting at the level of T11 segment. After modeling, the rats were randomly and equally divided into control group（n=18） and observation group（n=18）. 1 week after injury, DMEM/F12 medium 0.1 ml was injected into tail vein of rats in the control group, and an equal volume of BMSCs suspension was injected in the observation group. 1, 2, 4, 8 weeks after SCI, the hindlimb function was evaluated by the Basso- Beattie- Bresnahan（BBB） score. 2, 4, 8 weeks after SCI, the expression of GAP-43 m RNA was detected by RT-PCR, and the expression of GAP-43 was detected by the immunohistochemistry. Results 2, 4, 8 weeks after SCI, compared with the control group, The BBB score increased, the expression of GAP-43 m RNA and GAP-43 increased in the observation group（P〈0.05）. Conclusion BMSCs transplantation can improve the expression of GAP-43 in gene and protein level, and then promote the repair of SCI.