特发性肺纤维化患者外周血中CD4^+Foxp3^+调节性T细胞表达水平分析

Expression levels of CD4^+Foxp3^+ regulatory T cells in the peripheral blood of patients with idiopathic pulmonary fibrosis

目的探讨特发性肺纤维化患者外周血中Foxp3+调节性T细胞(Treg)的表达水平。方法选取36例特发性肺纤维化患者和40例健康者作为对照,分别采用流式细胞术、RT-PCR和ELISA法检测外周血CD4^+Foxp3^+Treg细胞百分率、核转录因子叉头翼状螺旋转录因子3(Foxp3)mRNA的表达以及血浆转化生长因子-β1(TGF-β1)的水平。结果特发性肺纤维化患者外周血中CD4^+Foxp3^+Treg细胞百分率(6.52±1.74)%、Foxp3 mRNA表达水平(0.38±0.16)及血浆TGF-β1水平(29 470±5 837)ng/L与正常对照组[(9.76±2.17)%、0.62±0.27、(38 510±6 724)ng/L]相比明显降低,差异有统计学意义(P<0.05)。结论特发性肺纤维化患者外周血中CD4^+Foxp3^+Treg细胞数量和功能异常,可能与特发性肺纤维化的发生有关。

Objective To explore the expression of CD4＋Foxp3＋regulatory T cells in the peripheral blood of patients with idiopathic pulmonary fibrosis. Methods A total of 36 patients with idiopathic pulmonary fibrosis and 40 normal controls were enrolled. The percentage of CD4＋Foxp3＋Treg cells,the mRNA level of foxhead winged-helix box protein 3（ Foxp3） and the levels of plasma transforming growth factor-β1（ TGF-β1）were detected by flow cytometry,real-time quantitative reverse transcription polymerase chain reaction（ RTPCR） and enzyme linked immunosorbent assay（ ELISA）. Results The percentage of CD4~＋Foxp3~＋Treg cells[（ 6. 52 ± 1. 74） % ],the mRNA level of Foxp3（ 0. 38 ± 0. 16） and the plasma levels of TGF-β1（ 29 470 ± 5837） ng / L in the peripheral blood of patients with idiopathic pulmonary fibrosis were statistically lower than those in normal controls [（ 9. 76 ± 2. 17） %,0. 62 ± 0. 27,（ 38 510 ± 6 724） ng / L ]（ P 0. 05）.Conclusions The number and function of CD4~＋Foxp3~＋Treg cells in peripheral blood of patients with idiopathic pulmonary fibrosis were abnormal. CD4~＋Foxp3~＋Treg cells may participate in the pathology of idiopathic pulmonary fibrosis.