纳豆菌液体发酵转化黄芩苷和汉黄芩苷的工艺研究

Transformation of baicalin and wogonoside through liquid fermentation with Bacillus natto

探索研究纳豆菌液体发酵黄芩药材制备黄芩素和汉黄芩素的工艺,利用纳豆菌所产生的活性酶对黄芩苷和汉黄芩苷进行生物转化,以提高黄芩中黄芩素和汉黄芩素的含量。通过考察不同碳源、氮源及无机盐的种类和浓度、培养基p H、药材粉碎度、装液量、摇床转速、液料比、发酵时间等因素对发酵工艺的影响,以黄芩素和汉黄芩素的含量为评价指标,优选得到黄芩生物转化生成黄芩素和汉黄芩素的液体发酵最佳工艺为蛋白胨质量分数为1.0%,氯化钠0.05%,p H 6.0,黄芩药材粉碎过40目,装样量33%,摇床转速200 r·min^(-1),液料比5∶1,温度37℃,发酵6 d,黄芩药材中黄芩苷的转化率为97.6%,汉黄芩苷转化率97.0%。验证试验表明该工艺稳定可行,为黄芩素和汉黄芩素的工业化生产提供数据参考。

This experiment aimed to explore and research the process of preparing baicalein and wogonin through liquid fermentation with Bacillus natto. Active enzymes of produced by B. natto was used for the biological transformation of baclin and wogonoside,in order to increase the content of the baicalein and wogonin in the scutellaria. With the content of the baicalein and wogonin as evaluating indexes,the effects of carbon source,nitrogen source,the types and suitable concentration of inorganic salt,medium p H,granularities of medical materials,liquid volume in flask,shaking speed,liquid-to-solid ratio,fermentation time on the fermentation process were studied. The optimal process conditions for liquid fermentation of scutellaria were 1. 0% of peptone,0. 05% of Na Cl,p H at 6,the granularities of medical materials of the scutellaria screened through 40-mesh sifter,33% of liquid,shaker incubator speed at 200 r·min^（-1）,liquid-to-solid ratio of 5∶ 1,temperature at 37 ℃,fermentation for 6 days,baclin＇s conversion rate at 97. 6% and wogonoside＇s conversion rate at 97% in the scutellaria. According to the verification test,the process was stable and feasible,and could provide data reference for the industrial production.