白茅内生菌Chaetomium globosum的化学成分研究

Chemical constituents from endophyte Chaetomium globosum in Imperata cylindrical

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等方法对白茅内生菌Chaetomium globosum固体发酵产物的化学成分进行分离纯化,获得9个化合物。利用高分辨质谱、核磁共振谱及文献比对的方法,将上述化合物分别鉴定为chaetoglobosin F(1),chaetoglobosin Fex(2),chaetoglobosin E(3),cytoglobosin A(4),penochalasin C(5),isochaetoglobosin D(6),N-benzoylphenylalaninyl-N-benzoylphenylalaninate(7),尿嘧啶(8),5-甲基尿嘧啶(9),其中化合物7为首次从毛壳菌中分离得到。采用MTT法测定化合物的体外细胞毒活性,结果显示,化合物1,3,4,5对人宫颈癌细胞株He La具有明显的抑制活性,IC50分别为99.43,23.77,97.92,86.25μmol·L^(-1),而阳性对照药顺铂的IC50为24.33μmol·L^(-1)。

Isolation and purification of chemical constituents from solid culture of endophyte Chaetomium globosum in Imperata cylindrical was performed through silica gel column chromatography,gel filtration over Sephadex LH-20 and preparative HPLC. Nine compounds were obtained and their structures were determined as chaetoglobosin F（ 1）,chaetoglobosin Fex（ 2）,chaetoglobosin E（ 3）,cytoglobosin A（ 4）,penochalasin C（ 5）,isochaetoglobosin D（ 6）,N-benzoylphenylalaninyl-N-benzoylphenylalaninate（ 7）,uracil（ 8）and 5-methyluracil（ 9）,respectively,based on HR-MS and NMR data and comparison with literatures. Compound 7 was isolated from Chaetomium sp. for the first time. In vitro cytotoxicity of compounds was evaluated using MTT mothed and 1,3,4 and 5 showed inhibition activity to the human cervical carcinoma cell He La with IC50 values of 99. 43,23. 77,97. 92,86. 25 μmol·L^（-1）,while positive control cisplatin had an IC50 value of 24. 33 μmol·L^（-1）.