摘要
The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A(one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing Con A or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the m RNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4^+ and CD8^+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.
The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa （GFP） by using mouse peritoneal macrophage and cytoxan （CTX） induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A （one component from GFP） stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing ConA or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the mRNA levels of IL-1β, IL-2, IL-6 and IFN-γ, in splenocyte. And GFP-A also significantly increased the expression of CD4^＋ and CD8^＋ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.
基金
supported by Key Projects in National Science&Technology Pillar Program during the 12th Five-Year Plan Period(No.2012BAD33B04)
Quality of Commodity Grain and Oil Resources and Processing Database Research–Grains(No.201313006)
Key Projects of Tianjin Natural Science Foundation(No.13JCZDJC29800)
863 National Plan(No.2012 AA022108)
Agricultural Science and Technology Transformation Fund(No.2012GB2A100016)
Plan for the Development of Changjiang Scholars and Innovative Research Team(No.IRT1166)
National Nature Science Foundation of China(Nos.31000768 and 31171731)
Tianjin Science and Technology Support Plan(No.10ZCZDSY 07000)
