摘要
The present study was designed to isolate and purify chemical constituents from solid culture of endophyte Aspergillus terreus LQ, using silica gel column chromatography, gel filtration with Sephadex LH-20, and HPLC. Fumigaclavine I(1), a new alkaloid, was obtained, along with seven known compounds, including fumigaclavine C(2), rhizoctonic acid(3), monomethylsulochrin(4), chaetominine(5), spirotryprostatin A(6), asperfumoid(7), and lumichrome(8). The structure of compound 1 was elucidated by various spectroscopic analyses(UV, MS, 1D and 2D NMR). The in vitro cytotoxicity of compound 1 was determined by MTT assay in human hepatocarcinoma cell line SMMC-7721, showing weaker cytotoxicity, compared with cisplatin, a clinically used cancer chemotherapeutic agent.
The present study was designed to isolate and purify chemical＇ constituents from solid culture of endophyte Aspergillus terreus LQ, using silica gel column chromatography, gel filtration with Sephadex LH-20, and HPLC. Fumigaclavine I （1）, a new alkaloid, was obtained, along with seven known compounds, including fumigaclavine C （2）, rhizoctonic acid （3）, monomethylsulochrin （4）, chaetominine （5）, spirotryprostatin A （6）, asperfumoid （7）, and lumichrome （8）. The structure of compound 1 was elucidated by various spectroscopic analyses （UV, MS, 1D and 2D NMR）. The in vitro cytotoxicity of compound 1 was determined by MTT assay in human hepatocarcinoma cell line SMMC-7721, showing weaker cytotoxicity, compared with cisplatin, a clinically used cancer chemotherapeutic agent.
基金
financially supported by National Natural Science Foundation of China(Nos.21372191 and 31370079)
Jiangsu Province Natural Science Foundation(No.BK20130437)
