醇提五倍子对鳙鱼非特异性免疫功能及抗病能力的影响

Influence of Nonspecific Immunity and Disease Resistance to Aristichtys nobilis Using Gallnut Extracted by Alcohol

为了研究五倍子对鳙鱼抗病能力的影响,将600尾鳙鱼（Aristichtys nobilis）随机分为4组,分别饲喂含有0.00%（对照）、0.50%、1.00%、2.00%（m/m）醇提五倍子的饵料,于第15、30、45天采集血样,检测各项免疫学指标,并以嗜水气单胞菌为指示菌进行攻毒试验,观察机体抗病能力。结果发现：（1）第15天,各组溶菌酶（LSZ）浓度依次为0.30、0.32、0.32、0.35μg/mL,其中2.0%组显著高于对照组（P〈0.05）;（2）随着五倍子添加量增加,超氧化物歧化酶（SOD）活力随之升高,第45天,1.00%、2.00%组浓度达到130、138U/mL,与对照组（115U/mL）相比差异显著（P〈0.05）;（3）第15天,试验组血浆球蛋白（GLB）含量均高于对照组,第45天1.00%组与对照组差异显著（P〈0.05）;（4）经攻毒试验,1.00%组存活率最高,达46.67%,对照组存活率仅为13.33%（P〈0.05）。以上试验结果表明,醇提五倍子可提高鳙鱼非特异性免疫能力,且对嗜水气单胞菌引起的感染具有一定抵抗作用,其在饵料中添加量以0.50%~1.00%为宜。

In order to study the effects of basic diet on non-specific immunity and disease resistance of Aristichtys nobilis.The fish in each group were fed basal diets adding 0.00%（control）,0.50%,1.00%,2.00%（m/m）alcohol extraction gallnut,respectively.After 15,30 and 45dblood samples were collected and detected immunological indexes,Aeromonas hydrophila as indicator bacteria finished the toxicity test,and observed the body disease-resistant ability.The results showed that：（1）In each group lysozyme concentration were 0.30,0.32,0.32 and 0.35μg/mL at 15 d,the 2.00% group were significantly higher than control group（P〈0. 05）.（2）SOD activity increased with the increased gallnut,1.00% group and2.00% group compared with the control group were significantly different in 45 d,the concentrationsreached to 130 and 138U/mL（P〈0. 05）;（3）Plasma globulin content of test group were higher than the control group at 15 d.There was significantly different between 1.00% group and the control group at 45d（P〈0. 05）;（4）Through the attack drug test,the survival rate was the highest in 1.00% group,up to46.67%.By contrast,the survival rate in control group were only 13.33%（P〈0. 05）.The test results showed that gallnut which was extracted by alcohol could increase the non-specific immunity of Aristichtys nobilis,and had certain resistance to Aeromonas hydrophila,and the suitable amount to added is from0.50%to 1.00%.