采用GC-MS法分析炮制对南葶苈子脂肪油成分的影响

GC-MS determination of fatty oil in seeds of Descurainia sophia after processing

目的:采用气相色谱-质谱联用(GC-MS)方法,比较研究南葶苈子炮制前后脂肪油成分的变化,为其临床用药提供科学依据。方法:以石油醚为提取溶剂,采用索氏法提取南葶苈子脂肪油,用GC-MS技术对其进行分析鉴定,DB-5型石英毛细管柱(30 m×0.25 mm,0.25μm),氦气流速1.0 m L·min-1,程序升温;离子源为电子轰击源,电子能量70 e V,离子源温度230℃。面积归一化法测定各成分的相对含量。结果:南葶苈子生品中检测出42个组分,鉴定出其中39个成分,占总检出化合物的99.75%,不饱和脂肪油占70.45%,含有炮制品中不含有成分12个,占生品脂肪油成分10.45%;南葶苈子清炒品中检测出50个组分,鉴定出其中40个成分,占总检出化合物的99.30%,不饱和脂肪油占79.85%,含有生品中不含有成分12个,占炮制品脂肪油成分4.00%。结论:南葶苈子经过炮制,脂肪油的提取率是生品的2倍以上,炮制品中不饱和脂肪油成分比例升高,同时产生了一些新的成分或一些成分溶出率升高,本研究为临床合理用药提供参考,具有一定的指导意义。

Objective： To analyze fatty oil in seeds of Deseurainia sophia after processing determined by gas chromatography-mass spectrometry（ GC-MS） for providing an experimental basis for clinical application.Methods： Fatty oil was extracted by Soxhlet extraction. Analysis and identification were made by GC-MS. The components were quantitatively determined by a normalization method. Results： A total of 42 components in crude product of D. sophia seeds were detected by GC-MS and 39 compounds were identified by MS,accounting for99. 75% of total detected components. In the detected components,70. 45% were unsaturated fatty oils,and 12 chemical constituents（ 10. 45%） were not detected in the processed product. In the samples of processed product,50 components were detected and 40 compounds were identified by MS,accounting for 99. 30% of total detected components. Unsaturated fatty oils accounted for 79. 85%,and 12 chemical constituents（ 4. 00%） were not detec-ted in the crude product. Conclusion： In the processed product,the total contents of unsaturated fatty oils increase,new chemical constituents are detected,and the solubility of some compounds may increase. These results will provide a reference for clinical use of seeds of D. sophia.