肺癌肿瘤抑制因子-1在喉鳞癌组织中的表达及其意义

Expression of tumor suppressor in lung cancer 1 in laryngeal squamous cell carcinoma and its significance

目的研究肺癌肿瘤抑制因子-1（TSLC1）在喉鳞癌组织和癌旁正常组织的表达,探讨TSLC1与喉鳞癌的相关性,以期为喉癌的病因、诊断及治疗提供理论依据。方法取喉鳞状细胞癌组织标本蜡块45例,其中8例有淋巴结转移。取20例癌旁正常组织标本蜡块作为对照。应用免疫组化PV-6000二步法测定二者中TSLC1的表达,分析TSLC1的表达水平与各临床病理参数的关系。结果用SPSS 10.0统计软件进行统计学分析。结果 TSLC1在喉鳞癌标本中的阳性表达率为17/45（37.8%）,明显低于癌旁正常组织中TSLC1的阳性表达率15/20（75.0%）,二者比较差异有统计学意义（χ~2=7.68,P〈0.01）。TSLC1的表达下降与喉鳞癌的临床分期、分化程度、淋巴结转移情况差异有统计学意义（χ~2=4.15、6.11、4.11,P〈0.05）,而与年龄、发病部位及性别差异无统计学意义（χ~2=0.25、0.09、1.0,P〉0.05）。结论 TSLC1在喉鳞癌中表达下降,且与喉鳞癌的临床分期、分化程度、淋巴结转移情况有相关性。TSLC1在喉鳞癌中的表达下降可能与喉鳞癌的发生和侵袭有关,但需扩大样本进一步研究。

Objective To investigate the correlation of tumor suppressor in lung cancer 1 （TSLC1）and laryngeal squa-mous cell carcinoma,in order to provide a theoretic basis of oncogenesis,diagnosis and treatment of laryngeal cancer. Methods Paraffin-embedded specimens were collected from 45 cases of laryngeal squamous cell carcinoma,8 of which had lymphatic metastasis.Another 20 specimens from normal adjacent tissues served as controls.The expression of TSLC1 was detected with immunohistochemical staining （PV-6000）.The relation between TSLC1 expression and other clinicopathological parameters was analyzed with SPSS 10.0.Results The positive expression of TSLC1 was 17 /45 （37.8%）in specimens of laryngeal squamous cell carcinoma,which was significantly lower than in specimens of normal adjacent tissues （15 /20,75%）,（χ2 =7.68,P 〈0.01 ）.Loss of TSLC1 expression was correlated with the clinical stage,tumor differentiation and lymph node metastasis （χ2 =4.15,6.11,4.11,P 〈0.05 ）,but not correlated with patients’age,gender,or location of primary laryngeal carcinoma （χ2 =0.25,0.09,1.0,P 〉0.05）. Conclusion Loss of TSLC1 expression is frequently observed in laryngeal squamous cell carcinoma,which is signifi-cantly correlated to the clinical stage,tumor differentiation and lymph node metastasis.The down-regulation of TSLC1 expression may correlate with the occurrence and invasion of laryngeal squamous cell carcinoma,but more studies are needed to verify the hypothesis.