邻香草醛缩胱氨酸双Schiff碱的合成及其对粟酒裂殖酵母细胞生长代谢的热动力学研究

SYNTHESIS AND THERMOKINETIC STUDY ON THE S.POMBE GROWTH METABOLISM OF A NOVEL BIS-SHCIFF BASE DERIVED FROM O-VANILLIN AND L-CYSTINE

用摩尔比为2:1的邻香草醛(C_8H_8O_3)与L-胱氨酸(C_6H_(12)N_2O_4S_2)反应,合成了一种新的双Schiff碱化合物--双{2-[(3-巯基丙酸钠)-2-亚胺基-甲基]-6-甲氧基-苯酚}(OVCS)。通过元素分析、红外光谱、核磁共振等手段对其组成和结构进行了表征,确定其化学式为Na_2(C_(22)H_(22)N_2O_8S_2),采用TAM air微量热仪测定了新合成的Schiff碱化合物(OVCS)在305.15 K时对粟酒裂殖酵母细胞作用的产热曲线;根据产热曲线计算了在OVCS作用下,粟酒裂殖酵母细胞生长代谢的最大发热功率p_(max)、速率常数k、传代时间tG、抑制率I和半抑制浓度C_(I,50)等热动力学参数。通过实验可以发现随着OVCS浓度的增加,粟酒裂殖酵母细胞的生长代谢速率常数k、生长代谢的总热效应Q_(total)、最大发热功率p_(max)均减小,抑制率I、达到生长代谢最大功率所需时间t_(max)、传代时间tG均增加等规律,半抑制浓度C_(I,50)为35.99 mg/L(或9.62×10^(-2)mol/L)。实验结果表明,OVCS对粟酒裂殖酵母细胞有抑制作用,且浓度越大,抑制作用越强。

A novel Schiff base named Bis{2-[（3-mercapto propionic acid sodium-2-ylimino）-methyl]-6-methoxy-phenol}（abbreviated as OVCS） was synthesized using 2：1 molar ratio of o-vanillin（C8H8O3） and L-cystine（C6H12N2O4S2）.Elemental analysis,IR and NMR spectroscopy were employed to characterize the composition and structure of OVCS.The analytical results show that the molecular formula of the Schiff base is Na2（C22H22N2O8S2）.The thermokinetic properties of the action of OVCS on the growth metabolism of Schizosaccharomyces pombe were investigated by a TAM air isothermal calorimetry at 305.15 K.Some thermokinetic parameters of growth metabolism of S.pombe,such as the maximum heating power（Pmax）,growth rate constant（k）,generation time（tG）,inhibition rate（I） and half inhibitory concentration（CI,50） were calculated.Experimental results indicate that the values of k,Qtotal and Pmax of S.pombe decrease,while the value of I,tmax and tG of S.pombe increased with the increase of the concentrations of OVCS.The CI,50 value of OVCS was found to be 35.99 mg/L（or 9.62×10^-2 mol/L）.From this work,one can reach a conclusion that OVCS possesses inhibitory effect on the growth metabolism of S.pombe,and the greater the concentration,the stronger the inhibition.