伴糖尿病的牙周炎患者全唾液中炎症因子水平的临床应用研究

Detection of Concentration of IL-6 in Whole Saliva in Patients with Periodontitis Accompanied with Diabetes and Its Significance

为比较糖尿病合并牙周炎患者、单纯糖尿病患者、单纯牙周炎患者以及健康者全唾液白细胞介素-6(IL-6)水平,及其与血糖控制情况和牙周病炎严重程度之间的关系,文章采用病例对照研究,纳入糖尿病合并牙周炎患者、单纯糖尿病患者(DM组)、单纯牙周炎患者(CP组)以及健康人群各30例,收集受检者静息全唾液,采用酶联免疫吸附试验(ELISA)检测全唾液中IL-6水平。结果显示,DM+CP组全唾液中IL-6水平显著高于其他3组,差异极具统计学意义(P<0.01);全唾液中IL-6水平随牙周炎严重程度加重而增高,随血糖控制情况的不理想而增高,差异有统计学意义;全唾液中IL-6水平与探诊深度(PD)、探诊出血指数(BI)、附着丧失(AL)、缺失牙数均呈正相关,且相关程度由高到低依次为PD、BI、AL、缺失牙数,差异有统计学意义(P<0.05)。文章最终得出结论,糖尿病患者体内的微炎症状态可能与牙周炎症有关,全唾液中IL-6水平既受牙周炎症影响也受全身因素的调控。

In order to compare the level of Interleukin-6（IL-6）in whole saliva in diabetic patients with periodontitis,simplex diabetic patients,patients with simplex periodontitis and healthy subjects,and its relationships with the control on blood glucose and severity of periodontal disease,the paper used the case-control study.There were 30 cases of patients with diabetes accompanied with periodontitis in DM＋CP group,30 cases of simple diabetic patients（DM group）,30 cases of patients with simple periodontitis（CP group）and 30 cases of healthy people. The resting whole saliva of subjects was collected. The level of IL-6 in whole saliva was detected using enzyme-linked immunosorbent assay（ELISA）. The results showed that the level of IL-6 in whole saliva in DM ＋CP group was significantly higher than that in other three groups,and the difference has statistical significance（P0.01）.The level of IL-6 in whole saliva increased with the increasing severity of periodontitis,increased with not ideal control on blood glucose,and the difference has statistical significance.The level of IL-6 in whole saliva showed a positive correlation with probing depth（PD）,probing bleeding index（BI）,attachment loss（AL）and number of missing teeth.Moreover the degrees of correlation from high to low were successively PD,BI,AL and the number of missing teeth.The difference was statistically significant（P 0.05）. The paper drew the conclusions： Micro inflammatory state in patients with diabetes may be associated with periodontal inflammation. The level of IL-6 in whole saliva was affected by both periodontitis and systemic factors.