铁皮石斛叶片内生真菌多样性的研究

Diversity of Endophytic Fungi in Leaves of Dendrobium candidum

为检测铁皮石斛内生真菌种类,采用高通量测序技术测定铁皮石斛内生真菌的ITS2区序列,用Qiime和Mothur等软件整理和统计样品序列数目和操作分类单元(OTUs)数量,分析物种的丰度和Alpha多样性。获得的有效序列和OTU数为41361/33;稀疏曲线表明测序深度充分,OTU的数量接近于饱和。铁皮石斛内生真菌主要分布于裸胞壳属Emericella,构巢裸壳孢菌Emericella nidulans是铁皮石斛内生真菌的优势种群。

To determine the diversity of the endophytic fungi grown on Dendrobium candidum,the ITS2 region from the samples was sequenced using the Illumina MiSeq high-through put sequencing method.The number of sequences and operational taxonomic units（OTUs）of each sample were sorted and calculated using Qiime and Mothur software.The species abundance and distribution,alpha diversity index,and time of species abundance of the fungi were determined.The number of effective sequences was found to be 41 361;and the OTUs was 33.The rarefaction functions indicated an adequate sampling,while the OTU number was close to saturation.It was concluded that the endophytic fungi found in D.candidumbelonged to Emericella,and E.nidulans was the dominant species.