内毒素在小鼠肝癌发生过程中的作用

Role of endotoxin in pathogenesis of hepatocellular carcinoma in mice

目的探讨内毒素在二乙基亚硝铵(DEN)诱发小鼠肝癌过程中的作用。方法四组雄性小鼠均喂食致癌物DEN液,A组为无菌小鼠,B组无菌小鼠腹腔注射内毒素,C组为普通小鼠,D组普通小鼠腹腔注射抗内毒素Ig Y。观察肝癌发生率和血清内毒素水平。结果内毒素水平最高的B组和C组肝癌发生率最高,内毒素水平最低的A组和D组肝癌发生率也最低,内毒素水平和肝癌发生率呈显著正相关。结论细菌的代谢产物内毒素是引起肝癌的根本原因。

Objective To explore the role of endotoxin in HCC induced by the DEN. Methods Four groups of male mice were fed with DEN. Group A is the germ free mice, group B is also the germ free mice injected by endotoxin in abdominal cavity, group C is normal bacte- rial mice, group D is also normal bacterial mice injected by anti - endotoxin IgY in abdominal cavity. Results The highest level of endotoxin in B group and C group had the highest incidence of hepatocellular carcinoma, the lowest level of endotoxin in A group and D group, the incidence of HCC is lowest. Endotoxin levels and tile incidence of HCC was positively related. Conclusion Endotoxin is the root cause of liver cancer.