生态免疫肠内营养干预重症胰腺炎大鼠模型肠道屏障功能、全身炎症反应的影响

Effect of ecological immune enteral nutrition intervention on intestinal barrier function and systemic inflammatory response in rat models with severe pancreatitis

目的:研究生态免疫肠内营养干预重症胰腺炎大鼠模型肠道屏障功能、全身炎症反应的影响。方法:取SD雄性大鼠并分为对照组、胰腺炎组、干预组,干预组在建立重症胰腺炎模型后进行生态免疫肠内营养干预。检测3组大鼠的血清内毒素含量、肠黏膜机械屏障和生物屏障功能、胰腺和肝脏损伤程度、炎症反应程度。结果:(1)肠道屏障功能:胰腺炎组血清内毒素含量、肠道内大肠杆菌的数目均高于对照组,肠黏膜厚度、绒毛高度以及肠道内双歧杆菌、乳酸杆菌含量的数目均低于对照组,差异均具有统计学意义(P<0.05);干预组血清内毒素含量、肠道内大肠杆菌的数目均低于胰腺炎组,肠黏膜厚度、绒毛高度以及肠道内双歧杆菌、乳酸杆菌含量的数目均低于胰腺炎组,差异均具有统计学意义(P<0.05);(2)全身炎症反应:胰腺炎组血清中淀粉酶、谷草转氨酶、TNF-α、HMGB-1、MCP-1、IL-6、MIF的含量均高于对照组,干预组血清中淀粉酶、谷草转氨酶、TNF-α、HMGB-1、MCP-1、IL-6、MIF的含量均低于胰腺炎组,差异均具有统计学意义(P<0.05)。结论:生态免疫肠内营养干预能够改善重症胰腺炎大鼠的肠道屏障功能,缓解全身炎症反应程度并减轻胰腺和肝脏的损伤程度。

Objective： To study the effects of ecological immune enteral nutrition intervention on intestinal barrier function and systemic inflammatory response in rat models with severe pancreatitis. Methods： SD male rats were taken and divided into control group, pancreatitis group and intervention group, and after severe pancreatitis models were established, interven tion group received ecological immune enteral nutrition intervention. Serum endotoxin contents, intestinal mucosal mechanical barrier and biological barrier function, pancreas and liver injury degree and inflammation degree of three groups were detected. Results： （1） Intestinal barrier function： serum endotoxin content and the number of intestinal Escherichia coli of pancreatitis group were higher than those of control group, and intestinal mucosal thickness, villus height and the number of intestinal Bifidobacterium and Lactobacillus were lower than those of control group; serum endotoxin content and the number of intestinal Escherichia coli of intervention group were lower than those of pancreatitis group, and intestinal mucosal thickness, villus height and the number of intestinal Bifidobacterium and Lactobacillus were higher than those of pancreatitis group; （2） systemic inflammation, serum amylase, aspartate aminotransferase, TNF-α, HMGB-1, MCP-1, IL-6 and MIF contents of pancreatitis group were higher than those of control group, and serum amylase, aspartate aminotransferase, TNF-α, HMGB-1, MCP- 1, IL-6 and MIF contents of intervention group were lower than those of pancreatitis group. Conclusion.. Ecological immune enteral nutrition intervention can improve the intestinal barrier function, alleviate systemic inflammation degree and reduce pancreas and liver injury degree in rats with severe pancreatitis.