尘螨过敏儿童的PAI-1基因多态性分析

PAI-1 gene polymorphism of dust mite allergens in children

目的：探讨尘螨过敏儿童的纤溶酶原激活物抑制物-1（PAI-1）基因多态性及其意义.方法：选择广州市妇女儿童医疗中心2013年6月～2015年6月就诊的160例过敏儿童为研究对象,进行尘螨过敏检测试验,选择试验结果阳性的80例作为观察组,试验结果为阴性80例健康儿童作为对照组,应用等位基因特异性扩增多聚酶联（AS PCR）方法对两组行PAI-1基因多态性分析.酶联免疫吸附试验（ELISA）方法测定血浆PAI-1含量.结果：基因型频率吻合度检测表明两组基因型达到Hardy-Wein-berg平衡;观察组的PAI-1 4G/4G基因型携带频率明显高于对照组（P＜0.05）;观察组5G/5G基因型携带频率明显低于对照组（P＜0.05）,而4G等位基因携带频率高于对照组（P＜0.05）;观察组患者组内比较4G/4G者血浆PAI-1含量明显高于4G/5G和5G/5G者（均P＜0.05）.结论：PAI-1基因多态性可能是尘螨过敏的危险因素,深入了解PAI-1基因的结构和功能,可以指导临床医生预防和干预.

Objective： To explore PAI-1 gene polymorphism of dust mite allergens in children. Methods.. A total of 160 allergic children who were admitted in our hospital from June, 2013 to June, 2015 were included in the study and received dust mite allergy test. A total of 80 eases with the positive results served as the observation group, while 80 healthy children with the negative results served as the control group. ASPCR was used to analyze PAI-1 gene polymorphism. ELISA was used to detect the content of plasma PAI-1. Results： The detection of genotype frequency goodness fit showed that the genotypes in the two groups reached Hardy-Weinberg balance. Frequency of PAI 1 4G/4G genotype in the observation group was significantly higher than that in the control group （P〈0.05）. 5G/SG genotype frequency in the observation group was significantly lower than that in the control group （P〈0.05）, while 4G allele frequency was significantly higher than that in the control group （P 〈0.05）. The content of plasma PAI 1 in the observation group with 4G/4G was significantly higher than that in patients with 4G/5G and 5G/SG. Conclusions： PAI-1 gene polymorphism is probably the risk factor for dust mite allergy. Deep insight of the structure and function of PAI 1 can guide the clinician to prevent and intervene the dust mite allergy in children.