应用抗p30血清检验人类精斑的研究

IDENTIFICATION OF HUMAN SEMEN STAINS USING ANTI-p30 SERUM

作者用自制的抗P30血清做琼脂双向扩散试验,检验人类精斑,并采用随机抽样和盲法试验与精子检出法进行比较。结果表明,用抗P30血清确证精斑的敏感性和准确性均高于精子检出法,且不受精液中有无精子的影响,也不受阴道液和唾液的干扰,并能正确区别人类与动物精斑,是目前确证人类精斑的最好方法。

The present communication describes theidentification of human semen stains usingimmunodiffusion test with anti-p30 serum.In order to evaluate objectively the value ofthe immunodiffusion test for the identifica-tion. Random sampling and blind study wereused. The results indicated that the im-munodiffusion test was more accurate thansperm detection. It was proved that p30 wasa useful human semen marker especially insemen stains of azoospermia and vaseliga-tion. The vaginal secretion and saliva inmixed stains did not interfere with theresult of the immunodiffusion. The humansemen stains can be differentiated from theanimal semen stains with anti-p30 serum.Therefore, it is concluded that the demon-stration of p30 in a suspected stain is thebest method for human semen identificationup to now.