布地奈德对哮喘气道重塑大鼠骨桥蛋白和mRNA表达的影响

Effect of Budesonide on the Expression of Osteopontin of Asthma Airway Remodelin Rats

目的探讨布地奈德对气道重塑模型大鼠肺组织骨桥蛋白(OPN)表达的影响及其在哮喘气道重塑中的作用。方法大鼠于第1天和第8天腹腔注射卵清蛋白(OVA)/AI(OH)3,第15天起雾化吸入OVA激发,隔天1次,制备哮喘气道重塑幼年大鼠模型。布地奈德组在雾化吸入OVA前30 min,给予雾化布地奈德混悬液(2ml∶1mg)。正常对照组以生理盐水代替OVA。运用彩色医学图像分析系统测量大鼠支气管壁总面积和平滑肌面积,计算平滑肌厚度(Wam)和支气管壁厚度(Wat),通过反转录-聚合酶链(RT-PCR)测定各组大鼠肺组织中OPN的mRNA,运用免疫组化法测定各组大鼠OPN蛋白的表达,并对Wat、Wam与OPN的蛋白和mRNA表达进行相关性分析。结果哮喘组Wat及Wam显著高于对照组,布地奈德组较哮喘组显著降低(P均<0.01)。免疫组化检测结果提示,布地奈德组OPN蛋白的表达高于正常对照组,但显著低于哮喘组,差异具有统计学意义(P均<0.01)。RT-PCR检测结果显示,哮喘组OPN mRNA表达均高于正常对照组,布地奈德组组较哮喘组显著减弱,差异具有统计学意义(P均<0.01)。肺组织中Wat、Wam与OPN的蛋白、mRNA表达均呈正相关。结论布地奈德能显著抑制哮喘大鼠OPN的表达,缓解气道重塑。

Objective To explore the effects of budesonide on osteopontin expression of asthma airway remodelin Rats. Methods SD rats were intraperitoneal injection with ovalbumin for a rat asthma airway remodeling model from day15.Budesonide group rats aerosolized budesonide 30 min before exposed to aerosolized ovalbumin.Physiological saline were instead in Normal control group with for sensitization and challenge.Brochial wall thickness（Wat） and brochial smooth muscle thickness（Wam） were mearured by image analysis system. The mRNA expressions of OPN of lung homogenate were detected by reverse transcription-polymerase chain reaction（RT-PCR）.The protein expressions of OPN of lung homogenate were detected by immunohistochemistry（IHC）.Besides,correlative analysis between Wam/Wat and the expression of OPN was studied. Results Wat and Wam of rats in asthma group was higher than normal control group,while those of budesonide group significant lower than asthma group（P〈0.01,respectvely）.OPN expression detected by IHC was significant lower in budesonide group than asthma group,but higher than normal control group（P〈0.01,respectvely）.OPN expression detected by RT-PCR in asthma group was significant higher than normal control group,while those of budesonide group were significantly lower than asthma group（P〈0.01,respectvely）.There was significantly positive correlation between Wat and the protein/mRNA expression of OPN,also between Wam and the protein/mRNA expression of OPN. Conclusion Budesonide decreases the the expression of OPN, involves in airway remodeling in asthma.